Long-term effects of porcine small intestine submucosa on the healing of medial collateral ligament: A functional tissue engineering study
Long-term effects of porcine small intestine submucosa on the healing of medial collateral ligament: A functional tissue engineering study
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DOI：
10.1002/jor.20080
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发表时间：
2006-04-01
影响因子：
2.8
通讯作者：
Abramowitch, SD
中科院分区：
文献类型：
--
作者：
Liang, R;Woo, SLY;Abramowitch, SD
猪小肠粘膜下层（SIS）先前被证明可以增强愈合内侧副韧带（MCL）的机械性能，并且发现其组织形态学外观和V/I型胶原比例与正常MCL接近。我们假设，从长远来看，即 26 周，SIS 可以引导组织更好的新韧带形成，通过减少 V 型胶原来增加机械性能和增加胶原纤维直径。在 38 只兔子中通过手术造成右侧 MCL 的 6 朗姆间隙损伤，而对侧完整的 MCL 作为假手术对照。在一半的动物中，将一条 SIS 缝合到切断的末端。另一半则没有应用 SIS。横截面积（CSA）是用激光测微系统测定的。股骨-MCL-胫骨复合体在单轴拉伸下进行机械测试。通过 H&E、免疫荧光染色和透射电子显微镜 (TEM) 测定组织形态学。使用十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）测定V/I型胶原蛋白的比例。与未处理组相比，经过 SIS 处理的 MCL 的 CSA 减少了 28%，切线模量增加了 33%，拉伸强度增加了 50%（p < 0.05）。 TEM 显示经 SIS 处理的韧带中的胶原原纤维组直径较大，而未处理组的原纤维均匀较小。 H&E 染色显示，SIS 处理组中的胶原纤维染色更密集，沿纵轴排列，并有更多散布的纺锤形细胞。免疫荧光染色显示 V 型胶原蛋白信号较少，与未处理对照相比，V 型/I 型胶原蛋白比例降低 5% 证实了这一点 (p < 0.05)。这些发现延长了 12 周的短期结果，并支持猪 SIS 作为生物支架促进韧带愈合的潜力。 (c) 2006 年骨科研究学会。
Porcine small intestinal submucosa (SIS) was previously shown to enhance the mechanical properties of healing medial collateral ligaments (MCL), and the histomorphological appearance and collagen type V/I ratio were found to be close to those of normal MCL. We hypothesized that at a longer term, 26 weeks, SIS could guide a better organized neo-ligament formation, increasing mechanical properties and increasing collagen fibril diameters mediated by a reduction in collagen type V. A 6 rum gap injury in the right MCL was surgically created in 38 rabbits, while the contralateral intact MCL served as a sham-operated control. In half the animals, a strip of SIS was sutured onto the severed ends. In the other half, no SIS was applied. The cross-sectional area (CSA) was determined with a laser micrometer system. The femur-MCL-tibia complex was mechanically tested in uniaxial tension. Histomorphology was determined through H&E and immunofluorescent staining and transmission electron microscopy (TEM). Sodium-dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was used to determine collagen type V/I ratio. SIS-treated MCLs displayed a 28% reduction in CSA, a 33% increase in tangent modulus, and a 50% increase in tensile strength compared with the nontreated group (p < 0.05). TEM showed groups of collagen fibrils with larger diameters in the SIS-treated ligaments in comparison with uniformly small fibrils for the nontreated group. H&E staining showed more densely stained collagen fibers in the SIS-treated group aligned along the longitudinal axis with more interspersed spindle-shaped cells. Inimunofluorescent staining showed less collagen type V signals, confirmed by a 5% lower ratio of collagen type V/I compared with the nontreated controls (p < 0.05). The findings extend the shorter term 12-week results, and support the potential of porcine SIS as a bioscaffold to enhance ligament healing. (c) 2006 Orthopaedic Research Society.