Polysaccharides from Lycium barbarum leaves: Isolation, characterization and splenocyte proliferation activity
Polysaccharides from Lycium barbarum leaves: Isolation, characterization and splenocyte proliferation activity
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枸杞叶多糖的分离、表征和脾细胞增殖活性
DOI：
10.1016/j.ijbiomac.2012.05.025
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发表时间：
2012-11-01
影响因子：
8.2
通讯作者：
Liu, Anjun
中科院分区：
文献类型：
--
作者：
Liu, Huihui;Fan, Yanli;Liu, Anjun
枸杞果实中多糖的研究已引起人们的广泛关注，但枸杞叶中多糖的研究报道较少。从枸杞叶中分离得到4个水溶性多糖组分：LBP-Ⅰ、LBP-Ⅱ、LBP-Ⅲ和LBP-Ⅳ。LBP-II和LBP-IV在HPGPC上分别有一个对称的峰，平均分子量分别为9.39 × 10（4）Da和4.18 × 10（5）Da，紫外光谱和红外光谱分析表明这两个组分具有酸性多糖与多肽或蛋白质结合的特征。气相色谱分析表明，LBP-Ⅳ主要由鼠李糖、阿拉伯糖、木糖、葡萄糖和半乳糖组成，摩尔比为1.61：3.82：3.44：7.54：1.00;硫酸-咔唑法测定糖醛酸含量为47.68%（w/w）。LBP-IV的H-1和C-13 NMR谱也显示存在羧基碳和5个异头碳，并表明在该馏分中可能存在α-和β-异头构型。脾细胞增殖活性测定表明，LBP-Ⅳ能显著促进ConA或LPS刺激的脾细胞增殖，表明LBP-Ⅳ具有免疫刺激活性。(C)2012爱思唯尔有限公司版权所有。
The polysaccharides from the fruits of Lycium barbarum have received considerable attention in previous publication, but the polysaccharides from the leaves were rarely reported. In the present work, four water-soluble polysaccharide fractions: LBP-I, LBP-II, LBP-III and LBP-IV isolated from L barbarum leaves were purified through DEAE-Sephadex A-25. LBP-II and LBP-IV respectively showed one symmetrical peak on HPGPC with average molecular weight of 9.39 x 10(4) Da and 4.18 x 10(5) Da. UV and IR analysis of the two fractions showed the characteristics of acidic polysaccharides combined with polypeptides or proteins. GC analysis showed LBP-IV was mainly composed of rhamnose, arabinose, xylose, glucose and galactose with molar ratio of 1.61:3.82:3.44:7.54:1.00, and the uronic acid content was 47.68% (w/w) determined by sulfuric acid-carbazole method. H-1 and C-13 NMR spectra of LBP-IV also showed the presence of carboxyl carbon and five anomeric carbons, and suggested there may be both alpha- and beta-anomeric configurations in this fraction. Moreover, splenocyte proliferation activity assay showed that LBP-IV significantly enhanced the proliferation of splenocyte stimulated by ConA or LPS, indicating the fraction has the beneficial effect on immunostimulating activity. (C) 2012 Elsevier B.V. All rights reserved.