Characterization of a human basophil-like cell line (LAMA-84)
Characterization of a human basophil-like cell line (LAMA-84)
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DOI：
10.1046/j.1365-3083.1996.d01-84.x
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发表时间：
1996-07-01
影响因子：
3.7
通讯作者：
Hellman, L
中科院分区：
文献类型：
--
作者：
Blom, T;Nilsson, G;Hellman, L
LAMA-84 是一种人类白细胞系，建立后被描述为具有巨核细胞、红细胞和粒细胞特征，分析了各种分化标记物的表达。除了之前描述的一些表型特征之外，还发现该细胞系表达嗜碱性白细胞和肥大细胞特有的几种蛋白质的 mRNA。作者表明，LAMA-84 细胞表达肥大细胞类胰蛋白酶、蛋白聚糖核心蛋白、羧肽酶 A 以及高亲和力 IgE 受体 (Fc epsilon RI) 的 α 链和 β 链的 mRNA。作者研究了 LAMA-84 在无血清培养基中或经 DMSO 或 PMA 处理后分化的潜力。根据诱导因子，Fc ε RI α链的表面表达从细胞的 20% 增加到 35-50%，并且类胰蛋白酶的 mRNA 水平在无血清培养基中和 DMSO 处理后增加。发现 LAMA-84 表达 CD13、CDw17、CD29、CD33、CD40、CD45 和 CD117。此外，还检测到嗜酸性粒细胞/嗜碱性粒细胞标记物夏科-莱登晶体 (CLC) 蛋白和主要碱性蛋白 (MBP) 以及红细胞分化标记物 α-球蛋白的 mRNA。然而，作者仅观察到另一种红系分化标记物（血型糖蛋白）的痕量 mRNA、巨核细胞标记物 GPIIIa 的痕量，并且未检测到 GPIb α 水平。通过比较 LAMA-84 和表达嗜碱性粒细胞表型的第二种人类细胞系 (KU812) 中一组分化标记物的表达模式，很明显，这些细胞系是目前唯一被鉴定为具有嗜碱性特征的两种细胞系，它们具有大量的表型特征。
LAMA-84, a human leucocytic cell line, which upon establishment was described as having mega-karyocytic, erythroid and granulocytic characteristics, was analysed for expression of various differentiation markers. In addition to some of the previously described phenotypic characteristics, this cell line was found to express mRNA for several proteins characteristic for basophilic leucocytes and mast cells. The authors show that LAMA-84 cells express mRNA for the mast cell tryptase, the proteoglycan core protein, carboxypeptidase A and the alpha and beta chains of the high affinity IgE receptor (Fc epsilon RI). The authors examined the potential of LAMA-84 to differentiate in serum-free medium or after DMSO or PMA treatment. Depending on the inducing factor, surface expression of the Fc epsilon RI alpha-chain was increased from 20% to 35-50% of the cells and mRNA levels for tryptase were increased in serum-free medium and after DMSO treatment. LAMA-84 was found to express CD13, CDw17, CD29, CD33, CD40, CD45 and CD117. Furthermore, mRNA for the eosinophil/basophil markers Charcot-Leyden crystal (CLC) protein and the major basic protein (MBP), as well as the erythrocyte differentiation marker alpha-globin, was detected. However, the authors observed only trace amounts of mRNA for another erythroid differentiation marker (glycophorin), trace amounts of the megakaryocytic marker GPIIIa, and no detectable level of GPIb alpha. By comparing the expression pattern of a panel of differentiation markers in LAMA-84, and a second human cell line (KU812) expressing a basophil phenotype, it is evident that these cell lines, which presently are the only two cell lines identified with basophilic characteristics, share a large number of phenotypic characteristics.