Direct evidence of interaction of a green tea polyphenol, epigallocatechin gallate, with lipid bilayers by solid-state nuclear magnetic resonance
Direct evidence of interaction of a green tea polyphenol, epigallocatechin gallate, with lipid bilayers by solid-state nuclear magnetic resonance
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DOI：
10.1271/bbb.68.1743
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发表时间：
2004-08-01
影响因子：
1.6
通讯作者：
Nakayama, T
中科院分区：
文献类型：
--
作者：
Kumazawa, S;Kajiya, K;Nakayama, T
用固体P-31和H-2NMR研究了儿茶素表没食子儿茶素没食子酸酯（EGCg）与二肉豆蔻酰磷脂酰胆碱（DMPC）模型膜的相互作用。DMPC磷酸基团的P-31化学位移各向异性在添加EGCg时降低。[4-H-2]EGCg在DMPC脂质体中的4-位被氘代，其H-2NMR谱给出了具有比固相中小得多的四极分裂的氘核。这些P-31和H-2 NMR观测结果提供了直接的实验证据，表明EGCg分子与脂质双层相互作用。
The interaction of a tea catechin, epigallocatechin gallate (EGCg), with the model membrane of dimyristoylphosphatidylcholine (DMPC) was studied by solid-state P-31 and H-2 NMR. The P-31 chemical shift anisotropy of the DMPC phosphate group decreased on addition of EGCg. The H-2 NMR spectrum of [4-H-2]EGCg, which is deuterated at the 4-position, in the DMPC liposomes gave deuterium nuclei with much smaller quadrupole splittings than those in the solid phase. These P-31 and H-2 NMR observations provide direct experimental evidence that the EGCg molecule interacts with the lipid bilayers.