High-speed three-dimensional human retinal imaging by line-field spectral domain optical coherence tomography
High-speed three-dimensional human retinal imaging by line-field spectral domain optical coherence tomography
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DOI：
10.1364/oe.15.007103
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发表时间：
2007-06-11
期刊：
影响因子：
3.8
通讯作者：
Yasuno, Yoshiaki
中科院分区：
文献类型：
--
作者：
Nakamura, Yoshifumi;Makita, Shuichi;Yasuno, Yoshiaki
线场谱域光学相干断层扫描 (LFSDOCT) 已开发用于超高速三维 (3D) 视网膜成像。通过该技术，单帧成像的 A 线速率显着提高到 823,200 A 线/秒，连续帧成像的 A 线速率提高到 51,500 A 线/秒。连续帧成像的帧率为 201 fps。该 3D 采集速度比标准飞点 SD-OCT 的采集速度高出两倍以上。在本文中，针对体内视网膜测量优化了相机的积分时间，并通过引入瞳孔光圈来抑制由于眼部像差导致的横向分辨率的下降。由于最佳积分时间，可以显着抑制运动伪影。此外，还采用了瞳孔光圈来增强 OCT 图像的对比度，以消除眼部像差的影响。体内 3D 视网膜成像具有 256 个横截面图像（256 个 A 线/图像），在 1.3 秒内成功完成，相当于 0.8 体积/秒。在 112 μm 深度处测得的最大轴上系统灵敏度为 89.4 dB，组织中的轴向分辨率为 7.4 μm。结果表明，在超高速采集模式下，LF-SDOCT 与飞点 SD-OCT 相比可能具有灵敏度优势。(c) 2007 年美国光学学会。
Line-field spectral domain optical coherence tomography (LFSDOCT) has been developed for very high-speed three-dimensional ( 3D) retinal imaging. By this technique, the A-line rate significantly improved to 823,200 A-lines/ s for single frame imaging and 51,500 A- lines/ s for continues frame imaging. The frame rate at continues frame imaging is 201 fps. This 3D acquisition speed is more than two fold higher acquisition speed than the standard flying spot SD-OCT. In this paper, the integration time of the camera was optimized for the in vivo retinal measurement and the degradation of the lateral resolution due to the ocular aberrations was suppressed by introducing the pupil stop. Owing to an optimal integration time, the motion artifact can be significantly suppressed. Also a pupil stop was employed in order to enhance the contrast of the OCT image for the effect of ocular aberrations. The in vivo 3D retinal imaging with 256 cross- sectional images ( 256 A-lines/ image) was successfully performed in 1.3 seconds, corresponding to 0.8 volume/s. The maximum on-axis system sensitivity was measured to be 89.4 dB at a depth of 112 mu m with an axial resolution of 7.4 mu m in tissue. It is shown that LF- SDOCT might have a sensitivity advantage in comparison to the flying spot SD- OCT in the ultra high- speed acquisition mode.(c) 2007 Optical Society of America.