Recombinant epidermal growth factor-like domain-1 from coagulation factor VII functionalized iron oxide nanoparticles for targeted glioma magnetic resonance imaging.
Recombinant epidermal growth factor-like domain-1 from coagulation factor VII functionalized iron oxide nanoparticles for targeted glioma magnetic resonance imaging.
复制标题
来自凝血因子 VII 功能化氧化铁纳米粒子的重组表皮生长因子样结构域 1，用于靶向神经胶质瘤磁共振成像
DOI：
10.2147/ijn.s116980
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发表时间：
2016
影响因子：
8
通讯作者：
Zhang W
中科院分区：
文献类型：
--
作者：
Liu H;Chen X;Xue W;Chu C;Liu Y;Tong H;Du X;Xie T;Liu G;Zhang W
胶质瘤细胞的高度浸润性和侵袭性常常导致肿瘤边缘模糊，从而导致肿瘤切除不完全和肿瘤复发。胶质瘤的准确检测和精确勾画有助于术前勾画、手术计划和生存预测。在这项研究中，重组表皮生长因子样结构域-1，来自人凝血因子VII，缀合到氧化铁纳米粒子（IONP）的靶向胶质瘤磁共振（MR）成像。合成的EGF 1-EGFP-IONP在体外对组织因子（TF）阳性的U87 MG细胞和人脐静脉内皮细胞表现出良好的靶向能力，并且在体内对临床前胶质瘤模型表现出持续和有效的MR对比增强长达12 h，具有高靶向特异性。它们在临床转化和开发针对脑胶质瘤的靶向治疗诊断学方面具有巨大潜力。
The highly infiltrative and invasive nature of glioma cells often leads to blurred tumor margins, resulting in incomplete tumor resection and tumor recurrence. Accurate detection and precise delineation of glioma help in preoperative delineation, surgical planning and survival prediction. In this study, recombinant epidermal growth factor-like domain-1, derived from human coagulation factor VII, was conjugated to iron oxide nanoparticles (IONPs) for targeted glioma magnetic resonance (MR) imaging. The synthesized EGF1-EGFP-IONPs exhibited excellent targeting ability toward tissue factor (TF)-positive U87MG cells and human umbilical vein endothelial cells in vitro, and demonstrated persistent and efficient MR contrast enhancement up to 12 h for preclinical glioma models with high targeting specificity in vivo. They hold great potential for clinical translation and developing targeted theranostics against brain glioma.