EBV-GENOME-NEGATIVE CELL LINE ESTABLISHED FROM AN AMERICAN BURKITT-LYMPHOMA - RECEPTOR CHARACTERISTICS - EBV INFECTIBILITY AND PERMANENT CONVERSION INTO EBV-POSITIVE SUBLINES INVITRO INFECTION
EBV-GENOME-NEGATIVE CELL LINE ESTABLISHED FROM AN AMERICAN BURKITT-LYMPHOMA - RECEPTOR CHARACTERISTICS - EBV INFECTIBILITY AND PERMANENT CONVERSION INTO EBV-POSITIVE SUBLINES INVITRO INFECTION
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DOI：
10.1159/000149930
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发表时间：
1975-01-01
期刊：
影响因子：
4.6
通讯作者：
MUMFORD, D
中科院分区：
文献类型：
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作者：
KLEIN, G;GIOVANELLA, B;MUMFORD, D
体外细胞系源自美国人伯基特淋巴瘤，命名为 Ra no.1。 1，当接种到胸腺缺陷的裸鼠体内时，会产生恶性肿瘤。这些细胞具有 B [骨髓来源]-淋巴细胞特征，具有表面相关的 .mu。和.kappa。链和 Epstein-Barr 病毒 (EBV) 受体，并且在体外很容易被 EBV 感染。 B95-8 病毒在 Ra 号中诱导 EBV 确定的核抗原 (EBNA)，但不诱导早期抗原 (EA)。 1细胞，而P3HR-1病毒诱导EBNA和EA； EA 水平低于原型 Raji 菌株。 Ra 中的 EBNA 和 EA 水平相当。 1 以及之前建立的 EBV 感染敏感 BJA-B 淋巴瘤。这两个品系有所不同，因为 BJA-B 可以被 B95-8 转化为永久携带 EBV 的品系，但不能被 P3HR-1 病毒株转化，而 Ra no。 1可以被P3HR-1病毒转化。这表明不同的 B 淋巴细胞来源的淋巴瘤系对同一病毒株的限制性控制可能有所不同。病毒株对同一宿主细胞的限制的敏感性各不相同。 Ra 号的永久 EBV 转换。根据 P3HR-4 病毒重复感染诱导的细胞对病毒抗原（EA，病毒衣壳抗原）的敏感性不变来判断，1 号细胞似乎没有改变细胞对照。
An in vitro line was derived from an American human Burkitt lymphoma, designated Ra no. 1, which produced malignant tumors when inoculated into thymus-deficient nude mice. The cells have B [bone marrow-derived]-lymphocyte characteristics, with surface-associated .mu. and .kappa. chains and Epstein-Barr virus (EBV) receptors, and can be readily infected with EBV in vitro. B95-8 virus induced EBV-determined nuclear antigen (EBNA) but not early antigen (EA) in Ra no. 1 cells, whereas P3HR-1 virus induced EBNA and EA; the EA level was lower than in the prototype Raji strain. EBNA and EA levels were comparable in Ra no. 1 and in the previously established, EBV-infection-sensitive BJA-B lymphoma. The 2 lines differed because BJA-B could be converted into a permanent EBV-carrying line by the B95-8 but not by the P3HR-1 virus strain, whereas Ra no. 1 could be converted by the P3HR-1 virus. This demonstrates that different B lymphocyte-derived lymphoma lines can vary with regard to the restrictive control they can exert on the same virus strain. Virus strains vary in their sensitivity to the restrictions of the same host cell. Permanent EBV conversion of the Ra no. 1 cell did not appear to change the cellular controls, as judged by the unchanged sensitivity of the cell to viral antigen (EA, viral capsid antigen) induction by P3HR-4 virus superinfection.