Dynamic Detection of Biogenic Amines as a Quality Indicator and Their Relationship with Free Amino Acids Profiles in Large Yellow Croaker (Pseudosciaena crocea)
Dynamic Detection of Biogenic Amines as a Quality Indicator and Their Relationship with Free Amino Acids Profiles in Large Yellow Croaker (Pseudosciaena crocea)
复制标题
大黄鱼质量指标生物胺的动态检测及其与游离氨基酸谱的关系
DOI：
10.1111/1750-3841.14425
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发表时间：
2019
影响因子：
3.9
通讯作者：
Fu Linglin
中科院分区：
文献类型：
--
作者：
Wang Yanbo;Bao Xingyue;Wang Feifei;Wang Haiyan;Fu Linglin
摘要生物胺（BA）的产生给海鲜保鲜带来了相当大的障碍。然而，人们对大黄鱼腐败特征的特征及其作用仍知之甚少。 4℃贮藏期间监测BAs含量、游离氨基酸(FAA)含量、需氧菌数、感官评价及多项特征理化指标。有力地揭示了腐胺和尸胺是主要的BAs，它们的总和可以代替BAI作为一种新的质量指标。建议不可接受的腐败的合理限制为 60 毫克/千克左右。 4℃时的回归模型（r>0.8，P<0.05）表明主要BAs与总挥发性碱氮（TVB-N）、蛋白质水解指数（P.I.%）以及pH值之间存在显着相关性，为水产品中BAs的理化性质提供了新的解释形式。此外，FAAs的演变表明赖氨酸、脯氨酸、组氨酸、甘氨酸和丙氨酸在整个贮藏过程中起主导作用，可以得出结论，大黄鱼体内BAs的生物合成与其前体物质存在中等程度的相关性。本工作对大黄鱼中BAs的来源进行了初步探讨。实际应用提出以腐胺和尸胺作为大黄鱼中主要BAs的总和值可以代替大黄鱼中的BAI作为一种新的质量指标。 BAs 和其他理化指标的回归模型为理解涉及 BAs 生物合成的腐败概况提供了新的视角。该研究可能有助于提高海鲜新鲜度的评估，并为海鲜保鲜提供新的靶点。
AbstractThe production of biogenic amines (BAs) set considerable obstacles in seafood preservation. However, the characteristic BAs and their roles in the spoilage profiles in large yellow croaker were still poorly understood. The BAs contents, free amino acids (FAAs) contents, aerobic bacterial count, sensory evaluation and several characteristic physicochemical indexes were monitored during storage at 4 °C. It's forcefully uncovered that the putrescine and cadaverine were the main BAs and the sum value of them could be used as a novel quality indicator instead of BAI. The reasonable limitation for unaccepted corruption was suggested be around 60 mg/kg. The regression model (r> 0.8,P< 0.05) at 4 °C indicated a significant correlation between the main BAs and total volatile basic nitrogen (TVB‐N), protein hydrolysis index (P.I.%) as well as pH value, offering a new interpretation form for the physiochemical properties of BAs in aquatic products. Additionally, the evolution of FAAs show the predominant role of lysine, proline, histidine, glycine, and alanine during the whole storage and it can be concluded that BAs biosynthesis in large yellow croaker were moderately related with their precursor substance. This work provides a preliminary insight into the origin of BAs in large yellow croaker.Practical ApplicationIt was proposed that the sum value of putrescine and cadaverine which were the main BAs can be used as a novel quality indicator instead of BAI in large yellow croaker. And the regression models of BAs and other physiochemical indexes provided a new perspective for comprehending the spoilage profiles involving BAs biosynthesis. The study may potentially contribute to improving the evaluation of seafood freshness and provide new target site for seafood preservation.