Immunohistochemical analysis of Marinesco bodies, using antibodies against proteins implicated in the ubiquitin-proteasome system, autophagy and aggresome formation
Immunohistochemical analysis of Marinesco bodies, using antibodies against proteins implicated in the ubiquitin-proteasome system, autophagy and aggresome formation
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使用针对泛素-蛋白酶体系统、自噬和聚集体形成中涉及的蛋白质的抗体对 Marinesco 小体进行免疫组织化学分析
DOI：
10.1111/j.1440-1789.2011.01267.x
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发表时间：
2012
期刊：
影响因子：
2.3
通讯作者：
et al.
中科院分区：
文献类型：
--
作者：
Odagiri S;et al.
Marinesco小体（MB）是黑质和蓝斑色素神经元中的球形嗜酸性核内包涵体。先前的免疫组织化学研究表明，MB对泛素，p62和SUMO-1呈阳性，表明泛素化和相关蛋白参与了MB的形成或解聚。然而，这种参与并没有被彻底理解。因此，我们用化学方法检查了5名年龄在53岁至84岁之间的对照受试者的中脑。MB对参与泛素-蛋白酶体系统（泛素、p62、EDD 1、NEDD 8、NUB 1、SUMO-1和SUMO-2）、侵袭体形成（HDAC 6）和自噬（泛素、p62、LC 3、GABARAP和GATE-16）的各种蛋白呈阳性。这些结果表明，与泛素化，蛋白酶体降解和自噬相关的蛋白质参与了MB的形成或解聚。
Marinesco bodies (MBs) are spherical eosinophilic intranuclear inclusions in pigmented neurons in the substantia nigra and locus ceruleus. Previous immunohistochemical studies have shown that MBs are positive for ubiquitin, p62 and SUMO‐1, suggesting the involvement of ubiquitination and related proteins in the formation or disaggregation of MBs. However, the involvement is not thoroughly understood. Therefore, we immunohistochemically examined the midbrain from five control subjects ranged from 53 to 84 years old. MBs were positive for various proteins implicated in the ubiquitin‐proteasome system (ubiquitin, p62, EDD1, NEDD8, NUB1, SUMO‐1 and SUMO‐2), aggresome formation (HDAC6) and autophagy (ubiquitin, p62, LC3, GABARAP and GATE‐16). These findings suggest that proteins related to ubiquitination, proteasomal degradation and autophagy are involved in the formation or disaggregation of MBs.