Preparation of encapsulated proteins dissolved in low viscosity fluids
Preparation of encapsulated proteins dissolved in low viscosity fluids
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DOI：
10.1023/a:1008354507250
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发表时间：
1999-05-01
影响因子：
2.7
通讯作者：
Wand, AJ
中科院分区：
文献类型：
--
作者：
Ehrhardt, MR;Flynn, PF;Wand, AJ
大多数蛋白质太大而无法通过溶液核磁共振方法进行全面检查，主要是因为它们在溶液中翻滚太慢。使大蛋白的 NMR 弛豫特性适合现代溶液 NMR 技术的一种潜在方法是将它们封装在溶解在低粘度流体中的反胶束中。不幸的是，有前途的低粘度流体，例如短链烷烃、超临界二氧化碳和各种卤化碳制冷剂，都需要施加很大的压力才能在室温下保持液化。在这里，我们描述了一种简单、经济有效的 NMR 管的设计和使用，该管适用于制备封装在溶解在此类液体中的反胶束中的蛋白质溶液。
The majority of proteins are too large to be comprehensively examined by solution NMR methods, primarily because they tumble too slowly in solution. One potential approach to making the NMR relaxation properties of large proteins amenable to modern solution NMR techniques is to encapsulate them in a reverse micelle which is dissolved in a low viscosity fluid. Unfortunately, promising low viscosity fluids such as the short chain alkanes, supercritical carbon dioxide, and various halocarbon refrigerants all require the application of significant pressure to be kept liquefied at room temperature. Here we describe the design and use of a simple cost effective NMR tube suitable for the preparation of solutions of proteins encapsulated in reverse micelles dissolved in such fluids.