Analysis of CCL5 expression in classical Hodgkin's lymphoma L428 cell line
Analysis of CCL5 expression in classical Hodgkin's lymphoma L428 cell line
复制标题
经典型霍奇金淋巴瘤L428细胞系中CCL5的表达分析
DOI：
10.3892/mmr.2011.515
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发表时间：
2011-09-01
影响因子：
3.4
通讯作者：
Zhao, Tong
中科院分区：
文献类型：
--
作者：
Liu, Fanrong;Zhang, Yan;Zhao, Tong
CCL 5是参与在弥漫性大B细胞淋巴瘤（DLBCL）和经典霍奇金淋巴瘤（CHL）中观察到的炎症的化学引诱细胞因子之一。然而，CCL 5的病理作用仍不清楚。为了更好地理解CCL 5在CHL和DLBCL中的作用，我们检测了CCL 5在CHL细胞系L428和DLBCL细胞系Ly 1和Ly 8中的表达，以及其对CD 4（+）T细胞的趋化作用。实时荧光定量RT-PCR检测CCL 5 mRNA的表达。用共聚焦显微镜分析细胞内CCL 5蛋白表达，用ELISA检测CCL 5蛋白分泌。采用Transwell共培养系统检测CCL 5的趋化功能，并计数迁移的CD 4（+）T细胞数。Western blotting检测NF-κ B α和NF-κ B信号分子p65的表达水平。结果显示，CCL 5在L428细胞中的mRNA和蛋白表达水平明显高于Ly 1和Ly 8细胞（p
CCL5 is one of the chemoattractant cytokines involved in inflammatory observed in both diffuse large B-cell lymphoma (DLBCL) and classical Hodgkin's lymphoma (CHL). However, the pathological effects of CCL5 remain unclear. To gain a better understanding of the role of CCL5 in CHL and DLBCL, we examined the expression of CCL5 in the CHL cell line L428 and the DLBCL cell lines Ly1 and Ly8, as well as its chemotactic effect on CD4(+) T cells. CCL5 mRNA expression was detected by real-time quantitative RT-PCR. Intracellular CCL5 protein expression was analyzed using confocal microscopy, and CCL5 protein secretion was detected by ELISA. The chemotactic function of CCL5 was assessed using a Transwell coculture system, and the number of migrated CD4(+) T cells was counted. Moreover, the p-i kappa B alpha and p65 levels of NF-kappa B signaling molecules in these lymphoma cell lines were detected by Western blotting. The results showed that CCL5 mRNA and protein expression in the L428 cells was significantly higher than in Ly1 and Ly8 cells (p