Aerobic degradation of 3,3′,4,4′-tetrachlorobiphenyl by a resuscitated strain Castellaniella sp. SPC4: Kinetics model and pathway for biodegradation
Aerobic degradation of 3,3′,4,4′-tetrachlorobiphenyl by a resuscitated strain Castellaniella sp. SPC4: Kinetics model and pathway for biodegradation
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DOI：
10.1016/j.scitotenv.2019.06.364
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发表时间：
2019-10-20
影响因子：
9.8
通讯作者：
Sun, Faqian
中科院分区：
文献类型：
--
作者：
Su, Xiaomei;Li, Si;Sun, Faqian
通过添加复苏促进因子（Rpf）获得的复苏菌株可以为获得高效的多氯联苯（PCB）降解菌提供绝大部分微生物来源。在本研究中，经Rpf复苏的casellaniella sp.菌株SPC4对3,3',4,4'-四氯联苯（PCB 77）的降解效率在复苏菌株和未复苏菌株中最高。进一步研究了复活菌株SPC4对PCB 77的降解能力，结果表明，在PCB 77浓度为20 mg/L时，SPC4能有效降解PCB 77，最大降解速率（q(max)）为0.066/h。在PCB 77上的最大生长速率为2.663 × 10(7) CFU/(mL.h) （0.024/h）。最适合的Edward模型表明，SPC4的q（max）为0.9315/h，底物亲和力为11.33 mg/L，底物抑制常数为11.41 mg/L。同时，bphA基因表达和氯离子释放的存在，以及代谢产物的鉴定，证实了bph编码的联苯途径参与了SPC4对KB 77的矿化。该报告首次证实了复苏菌株Castellaniella sp. SPC4对PCB 77的好氧降解，表明PCB生物修复具有良好的潜力。(C) 2019 Elsevier B.V.版权所有
Resuscitated strains which were obtained by addition of resuscitation promoting factor (Rpf) could provide a vast majority of microbial source for obtaining highly efficient polychlorinated biphenyl (PCB)-degrading bacteria. In this study, the Caslellaniella sp. strain SPC4 which was resuscitated by Rpf addition showed the highest efficiency in degradation of 3,3',4,4'-tetrachlorobiphenyl (PCB 77) among the resuscitated and non-resuscitated isolates. Further investigations on the PCB 77 degradation capability of the resuscitated strain SPC4 showed that SPC4 could efficiently degrade PCB 77 with maximum degradation rate (q(max)) of 0.066/h at about 20 mg/L of PCB 77. The maximum growth rate on PCB 77 was 2.663 x 10(7) CFU/(mL.h) (0.024/h). The most suitable model of Edward demonstrated that the SPC4 could achieve q(max) of 0.9315/h, with substrate-affinity of 11.33 mg/L and substrate-inhibition constants of 11.41 mg/L. Meanwhile, the presence of bphA gene expression and chlorine ions release, together with the identification of metabolites, confirmed that the bph-encoded biphenyl pathway was involved in KB 77 mineralization by SPC4. This report is the first to demonstrate aerobic degradation of PCB 77 by the resuscitated strain Castellaniella sp. SPC4, indicating excellent potential for PCB bioremediation. (C) 2019 Elsevier B.V. All rights reserved.