Capillary electrophoresis.
Capillary electrophoresis.
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DOI：
10.1021/ac00179a722
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发表时间：
1989
影响因子：
7.4
通讯作者：
中科院分区：
文献类型：
--
作者：
将毛细管电泳（CE）技术应用于地下水迁移示踪剂荧光素的激光诱导荧光（LIF）检测，并与传统荧光分光光度法进行了比较。在低万亿分之一（ppt）范围内的注入染料的检测限已完成与CE/LIF的基础上的Ar离子激光器和荧光分光光度计。这种方法被用于一个现实世界的问题，在确定相邻的资源保护和恢复法案（RCRA）和超级基金网站之间的地下水迁移的环境科学司在响应区域的需要，并作为正在开发的新的分析工具的应用。将荧光染料注入源威尔斯井，然后在监测威尔斯井中通过吸附染料的萃取垫或通过使用固相萃取（SPE）（一种预浓缩技术）直接测定水中的染料来测定。由于染料的分离和独特的迁移时间，基于CE/LIF的方法比现有方法表现出更高的特异性。另外的研究旨在使用固相萃取和场放大注射技术实现水中的亚ppt水平。
Capillary electrophoresis (CE) has been applied to the determination of the groundwater migration tracer dye fluorescein based on laser-induced fluorescence (LIF) detection and compared to determinations obtained with traditional spectrofluorimetry. Detection limits of injected dye in the low parts per trillion (ppt) ranges have been accomplished with both CE/LIF based on the Ar ion laser and with a spectrofluorimeter. This approach was used for a real-world problem in determining groundwater migration between adjacent Resource Conservation and Recovery Act (RCRA) and Superfund sites by the Environmental Sciences Division in response to regional needs and as application of new analytical tools under development. Fluorescent dye was injected into source wells and then was determined in monitoring wells by extracting pads that adsorbed the dye or by directly determining the dye in the water using solid-phase extraction (SPE), a preconcentration technique. The approaches based on CE/LIF exhibits increased specificity over existing approaches due to the separation and unique migration time of the dye. Additional studies were aimed at achieving sub-ppt levels in the water using solid-phase extraction and field-amplified injection techniques.