Additional Landing Sites for Recombination-Mediated Cassette Exchange in C. elegans.
Additional Landing Sites for Recombination-Mediated Cassette Exchange in C. elegans.
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DOI：
10.17912/micropub.biology.000503
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发表时间：
2021
影响因子：
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通讯作者：
Nonet M
中科院分区：
文献类型：
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作者：
Nonet M
重组介导的盒交换（RMCE）是最近开发的用于使用重组而不是双链断裂的修复作为驱动整合到基因组中的机制来产生单拷贝转基因的替代方法。已经开发了用于进行RMCE的两种替代方法;使用FLP重组酶的未连接来源的双组分方法，以及使用着陆位点内的FLP表达盒的单组分方法。在这里，我描述了用于执行这两种类型的RMCE的新着陆点。新的着陆位点位于Chr II和Chr IV上经过充分审查的MosSCI插入位点的50 bp内。
Recombination-mediated cassette exchange (RMCE) is a recently developed alternative method for creating single copy transgenes using recombination rather than repair of double stranded breaks as the mechanism for driving integration into the genome. Two alternative methods for performing RMCE have been developed; a two-component approach using an unlinked source of FLP recombinase, and a one-component approach using a FLP expression cassette within the landing site. Here, I describe new landing sites for performing both types of RMCE. The new landing sites are located within 50 bp of well-vetted MosSCI insertion sites on Chr II and Chr IV.