A BEHAVIORAL SCREEN FOR ISOLATING ZEBRAFISH MUTANTS WITH VISUAL-SYSTEM DEFECTS
A BEHAVIORAL SCREEN FOR ISOLATING ZEBRAFISH MUTANTS WITH VISUAL-SYSTEM DEFECTS
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DOI：
10.1073/pnas.92.23.10545
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发表时间：
1995-11-07
影响因子：
11.1
通讯作者：
DOWLING, JE
中科院分区：
文献类型：
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作者：
BROCKERHOFF, SE;HURLEY, JB;DOWLING, JE
研究了斑马鱼幼鱼的光动力学和趋光行为对筛选视觉系统隐性缺陷的作用。在5天龄的幼鱼中，光动力学反应可以可靠、快速地检测到，而幼鱼的光致反应是可变的，不足以用于筛选。因此，我们测量了诱变幼虫的光动力学反应，作为视觉系统缺陷的遗传筛选，检测了代表266个突变基因组的第三代幼虫的异常光动力学反应，鉴定了18个光动力学缺陷突变体，并进一步研究了2个没有明显形态缺陷的突变体，无光动力学反应a（noa）和部分光动力学反应a（pea）。我们记录视网膜电图（ERG）来确定这两种突变是否影响视网膜，noa幼虫的b波出现严重异常，开始延迟，振幅明显降低，而poa幼虫的ERG波形正常，虽然在强光下b波振幅降低，但从组织学上看，noa和poa幼虫的视网膜正常，我们认为noa幼虫在外层视网膜有功能缺陷。然而，poa幼虫的外视网膜可能是正常的。
Optokinetic and phototactic behaviors of zebrafish larvae were examined for their usefulness in screening for recessive defects in the visual system, The optokinetic response can be reliably and rapidly detected in 5-day larvae, whereas the phototactic response of larvae is variable and not robust enough to be useful for screening. We therefore measured optokinetic responses of mutagenized larvae as a genetic screen for visual system defects, Third-generation larvae, representing 266 mutagenized genomes, were examined for abnormal optokinetic responses, Eighteen optokinetic defective mutants were identified and two mutants that did not show obvious morphological defects, no optokinetic response a(noa) and partial optokinetic response a (pea), were studied further, We recorded the electroretinogram (ERG) to determine whether these two mutations affect the retina, The b-wave of noa larvae was grossly abnormal, being delayed in onset and significantly reduced in amplitude, In contrast, the ERG waveform of poa larvae was normal, although the b-wave was reduced in amplitude in bright light, Histologically, the retinas of noa and poa larvae appeared normal, We conclude that noa larvae have a functional defect in the outer retina, whereas the outer retina of poa larvae is likely to be normal.