A novel ex vivo skin model for the assessment of the potential transcutaneous anti-inflammatory effect of topically applied Harpagophytum procumbens extract.
A novel ex vivo skin model for the assessment of the potential transcutaneous anti-inflammatory effect of topically applied Harpagophytum procumbens extract.
复制标题
一种新型离体皮肤模型，用于评估局部应用的哈巴戈植物提取物的潜在经皮抗炎作用。
DOI：
10.1016/j.ijpharm.2009.04.017
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发表时间：
2009
影响因子：
5.8
通讯作者：
C. Heard
中科院分区：
文献类型：
--
作者：
Nassima Abdelouahab Ouitas;C. Heard
使用离体皮肤作为模型，这项工作测试的假设，即局部应用Harpagophytumprocumbens（H。平卧草）可以在经皮递送后在更深的组织中引发抗炎反应。采用Franz扩散池，对粉末H。将平卧块茎给药到新鲜切除的猪皮上。24小时后，回收受体相，分析DC的主要糖苷，然后直接用于进一步给药新鲜切除的皮肤膜。6小时后，回收皮肤，并使用免疫细胞化学和Western印迹分析探测参与炎症因子的三种主要酶的表达：环氧合酶（考克斯-2）及其产物前列腺素E2（PGE-2）、脂氧合酶（5-LOX）和诱导型一氧化氮（iNOS）。24 h受体相含哈巴俄苷、哈巴苷、毛蕊花苷、8-O-对香豆酰哈巴苷分别为（0.8，25，1.8，3×10−3）μmolmL− 1。当应用于皮肤时，该溶液有效地抑制了考克斯-2及其产物PGE-2的表达。然而，与对照样品（仅PBS）相比，它对5-LOX或iNOS没有显著影响。这些数据支持的假设，经皮输送H。平卧草可以治疗较深组织的炎症，如关节炎。此外，已经描述了一种新的离体模型，用于评估递送至更深皮下区域的渗透物的潜在抗炎活性。
Using ex vivo skin as a model, this work tested the hypothesis that the major pharmacologically active components of topically applied Harpagophytum procumbens (H. procumbens) can elicit anti-inflammatory responses in deeper tissues post-transcutaneous delivery. Using Franz-type diffusion cells, ethanol extract of powdered H. procumbens tuber was dosed onto freshly excised porcine skin. After 24h the receptor phase was recovered, analysed for the major glycosides of DC, then used directly to dose further freshly excised skin membranes. After 6h the skin was recovered and probed for the expression of the three major enzymes involved in the inflammatory factors: cyclooxygenase (COX-2) and its product prostaglandin E2 (PGE-2), lipoxygenase (5-LOX), and inducible nitric oxide (iNOS), using immunocytochemistry and Western blotting analyses. It was found that the receptor phase at 24h contained (0.8, 25, 1.8, 3×10−3)μmolmL−1of harpagoside, harpagide, verbascoside, 8-O-p-coumaroyl-harpagide, respectively. When applied to skin, this solution effectively inhibited the expression of COX-2 and its product PGE-2. However, it did not have a significant effect on either 5-LOX or iNOS compared to control samples (PBS only). These data support the hypothesis that the transcutaneous delivery of H. procumbens can treat inflammation in deeper tissues such as in arthritis. Moreover, a novel ex vivo model has been described for assessing the potential anti-inflammatory activity of permeants delivered to deeper subcutaneous regions.