A novel fluorescence aptasensor based on mesoporous silica nanoparticles for selective and sensitive detection of aflatoxin B1
A novel fluorescence aptasensor based on mesoporous silica nanoparticles for selective and sensitive detection of aflatoxin B1
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DOI：
10.1016/j.aca.2019.04.014
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发表时间：
2019-08-30
影响因子：
6.2
通讯作者：
Yu, Yong
中科院分区：
文献类型：
--
作者：
Tan, Hongxia;Ma, Liang;Yu, Yong
基于介孔二氧化硅纳米颗粒(MSN)，设计了一种新型、简单、无标记的适配子生物传感器，用于黄曲霉毒素B-1(AFB(1))的检测。在此，适体被用作分子识别探针和门控分子，而Rh6G被负载到粒子内部作为信号探针。在没有AFB(1)的情况下，由于适体被固定在MSN-NH2表面，关闭了“门”以防止信号探针的泄漏。在AFB(1)存在的情况下，可以打开“门”以释放信号探针，从而与AFB(1)特异性结合适体。结果表明，荧光强度与黄曲霉毒素B(1)的浓度呈正相关(0.5-50 ng mL(-1))，最低检出限为0.13 ng mL(-1)。更重要的是，该设计为基于适配子的快速、灵敏和选择性检测提供了一种新的方法，如果有合适的适配子可用，它可以应用于许多其他分析物。(C)2019爱思唯尔B.V.保留所有权利。
Based on the mesoporous silica nanoparticles (MSN), a novel, simple and label-free aptamer biosensor was designed for the detection of aflatoxin B-1 (AFB(1)). Here, the aptamers were used as molecular recognition probes and "gated molecules" while Rh6G was loaded into the interior of the particles as the signal probe. In the absence of AFB(1), the "gate" was closed to prevent the leakage of the signal probe because of the immobilization of aptamers on the surface of MSN-NH2. With the presence of AFB(1), the "gate" could be opened to release the signal probe for the specifical binding of aptamers to AFB(1). Our results showed that the fluorescence intensity was positively correlated with the concentration of AFB(1) (0.5-50 ng mL(-1)), with the detection limit as low as 0.13 ng mL(-1). What's more, this design provides a new approach for rapid, sensitive and selective detection based on aptamers and it could be applied to numerous other analytes if appropriate aptamers are available. (C) 2019 Elsevier B.V. All rights reserved.