Size control of giant unilamellar vesicles prepared from inverted emulsion droplets
Size control of giant unilamellar vesicles prepared from inverted emulsion droplets
复制标题
DOI：
10.1016/j.jcis.2012.02.029
复制
发表时间：
2012-06-15
影响因子：
9.9
通讯作者：
Yomo, Tetsuya
中科院分区：
文献类型：
--
作者：
Nishimura, Kazuya;Suzuki, Hiroaki;Yomo, Tetsuya
生产具有控制大小和结构的巨大脂质囊泡将是设计模拟细胞微室定量生物分析的重要技术。为了建立巨型囊泡的大小控制，我们研究了倒置的乳状液滴在通过油水界面时转化为巨型单层囊泡的形成过程。利用微流控制备的粒径分布较窄的倒置乳液液滴，研究了模板乳液尺寸与转化guv的关系。我们成功地找到了合适的离心加速条件，获得了理想尺寸和足够窄的尺寸分布，并提高了产率，使乳液液滴成为guv的模板。爱思唯尔公司版权所有版权所有。
The production of giant lipid vesicles with controlled size and structure will be an important technology in the design of quantitative biological assays in cell-mimetic microcompartments. For establishing size control of giant vesicles, we investigated the vesicle formation process, in which inverted emulsion droplets are transformed into giant unilamellar vesicles (GUVs) when they pass through an oil/water interface. The relationship between the size of the template emulsion and the converted GUVs was studied using inverted emulsion droplets with a narrow size distribution, which were prepared by microfluidics. We successfully found an appropriate centrifugal acceleration condition to obtain GUVs that had a desired size and narrow-enough size distribution with an improved yield so that emulsion droplets can become the template for GUVs. Crown Copyright (C) 2012 Published by Elsevier Inc. All rights reserved.