An immunohistochemical study of TIMP-3 expression in oesophageal squamous cell carcinoma.
An immunohistochemical study of TIMP-3 expression in oesophageal squamous cell carcinoma.
复制标题
DOI：
10.1038/sj.bjc.6602185
复制
发表时间：
2004-10-18
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
--
作者：
金属蛋白酶组织抑制因子-3(TIMP-3)抑制基质金属蛋白酶的活性，可能在肿瘤的侵袭和转移中起重要作用。探讨TIMP-3在食管鳞癌(ESCC)中的表达及其与临床病理因素的关系。我们检查了从1983至2001年间接受手术的90例胸段食道癌患者的组织标本。免疫组织化学染色采用链霉亲和素-生物素标记法。TIMP-3免疫染色见于癌细胞和正常食道上皮细胞的胞浆，尤其是位于肿瘤浅部的细胞。在90例患者中，TIMP-3保留(+)、中度(±)和减少(−)分别为30、27和33例(33、30、37%)。TIMP-3的表达与肿瘤侵袭深度(P=0.001)、有无淋巴结转移(P=0.003)、浸润性生长方式(P=0.003)和疾病分期(P=0.005)显著相关。TIMP-3(−)癌患者的生存率显著低于TIMP-3(+)和TIMP-3(±)癌(P=0.0003)。TIMP-3(+)、(±)和(−)患者的平均5年生存率分别为50、58和21%。综上所述，TIMP-3蛋白表达降低与肿瘤的侵袭活动和转移有关。这使得失去TIMP-3的癌症患者的预后明显不如保持TIMP-3的癌症患者。
Tissue inhibitor of metalloproteinase-3 (TIMP-3) inhibits the activity of matrix metalloproteinase, which may play an important role in carcinoma invasion and metastasis. We have investigated the relationship between TIMP-3 reduction and clinicopathological factors in oesophageal squamous cell carcinoma (ESCC). We examined tissue specimens that had been removed from 90 patients with thoracic oesophageal cancer who had undergone surgery between 1983 and 2001. Immunohistochemical staining was performed by the standard streptavidin–biotin method. Immunostaining of TIMP-3 was seen in the cytoplasm of cancer cells and normal oesophageal epithelial cells, particularly in cells located in shallow areas of the tumour. TIMP-3 preserved (+), moderate (±), and reduced (−) cases accounted for 30, 27, and 33 of the 90 patients, respectively (33, 30, 37%). Significant correlations were observed between TIMP-3 expression and depth of tumour invasion (P=0.001), number of lymph node metastases (P=0.003), infiltrative growth pattern (P=0.003), and disease stage (P=0.005). The survival rates of patients with TIMP-3 (−) cancer were significantly lower than those of patients with TIMP-3 (+) and TIMP-3 (±) cancer (P=0.0003). The mean 5-year survival rates of patients with TIMP-3 (+), (±), and (−) were 50, 58, and 21%, respectively. In conclusion, decreased expression of TIMP-3 protein correlates with invasive activity and metastasis. This makes the prognosis for patients with cancer that has lost TIMP-3 significantly less favourable than that for patients with cancer that has maintained TIMP-3.
登录
查看更多内容
影响因子：
4.7
作者：
Bian, JH;Wang, YL;Sun, Y
通讯作者：
Sun, Y
影响因子：
82.9
作者：
Qi, JH;Ebrahem, Q;Anand-Apte, B
通讯作者：
Anand-Apte, B
影响因子：
4.8
作者：
APTE, SS;OLSEN, BR;MURPHY, G
通讯作者：
MURPHY, G
影响因子：
20.3
作者：
Spurbeck, WW;Ng, CYC;Davidoff, AM
通讯作者：
Davidoff, AM
影响因子：
64.8
作者：
SATO, H;TAKINO, T;SEIKI, M
通讯作者：
SEIKI, M