Elimination of rat spinal neurons expressing neurokinin 1 receptors reduces bladder overactivity and spinal c-fos expression induced by bladder irritation.
Elimination of rat spinal neurons expressing neurokinin 1 receptors reduces bladder overactivity and spinal c-fos expression induced by bladder irritation.
复制标题
消除表达神经激肽 1 受体的大鼠脊髓神经元可减少膀胱过度活动和膀胱刺激引起的脊髓 c-fos 表达。
DOI：
10.1152/ajprenal.00274.2004
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Yoshimura,Naoki
中科院分区：
文献类型：
--
作者：
Seki,Satoshi;Erickson,KristinA;Seki,Masako;Nishizawa,Osamu;Igawa,Yasuhiko;Ogawa,Teruyuki;deGroat,WilliamC;Chancellor,MichaelB;Yoshimura,Naoki
Substance P (SP) binding to neurokinin 1 receptors (NK1R) in the spinal cord reportedly plays an important role in the micturition reflex as well as in nociceptive responses. We therefore investigated the effect of ablation of NK1R-expressing neurons in the spinal cord using saporin, a ribosome-inactivating protein, conjugated with [Sar9, Met (O2)11]SP, a specific ligand of NK1R (SSP-saporin), on the micturition reflex in rats. In female Sprague-Dawley rats, SSP-saporin (1.0 or 1.5 μM) or saporin (1.5 μM) only was injected through an intrathecal catheter implanted at the L6-S1level of the spinal cord. Three weeks after intrathecal administration of SSP-saporin, NK1R immunoreactivity in lamina I of the spinal cord was significantly reduced, but cystometric parameters in awake rats were not altered. Instillation of capsaicin (15 μM) into the bladder of normal rats induced bladder overactivity. This response to capsaicin was significantly suppressed in SSP-saporin-treated animals. SSP-saporin treatment also decreased c-fosexpression in the dorsal horn of the spinal cord induced by instillation of capsaicin into the bladder. These data indicate that NK1R-expressing neurons in the superficial layer of the dorsal horn play an important role in transmission of nociceptive afferent information from the bladder to induce bladder overactivity and spinal c-fosexpression elicited by bladder irritation. Toxin-induced damage of NK1R-expressing neurons in the lumbosacral spinal cord may provide an effective modality for treating overactivity and/or nociceptive responses in the bladder without affecting normal micturition.
