Myomaker, Regulated by MYOD, MYOG and miR-140-3p, Promotes Chicken Myoblast Fusion.
Myomaker, Regulated by MYOD, MYOG and miR-140-3p, Promotes Chicken Myoblast Fusion.
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DOI：
10.3390/ijms161125946
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发表时间：
2015-11-02
影响因子：
5.6
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Luo W;Li E;Nie Q;Zhang X
成肌细胞的融合是骨骼肌分化过程中的重要环节。最近在小鼠中的一项研究发现，一种名为Myomaker的跨膜蛋白，在肌肉中特异性表达，对成肌细胞融合至关重要。然而，其作用的细胞机制和其表达的调控机制仍不清楚。鸡不仅在肉类生产中占有重要地位，而且是研究肌肉发育的理想模式生物。在这里，我们报告，Myomaker也是鸡成肌细胞融合所必需的。Myomaker在鸡原代成肌细胞中的强制表达促进成肌细胞融合，而通过siRNA敲低Myomaker抑制成肌细胞融合。MYOD和MYOG属于肌源性调节因子家族，可与位于Myomaker转录起始位点附近的保守E-box结合并诱导Myomaker转录。此外，miR-140- 3 p可以通过在体外结合Myomaker的3个UTR来至少部分地抑制Myomaker表达和成肌细胞融合。这些发现证实了Myomaker在禽类成肌细胞融合中的重要作用，并表明MYOD、MYOG和miR-140- 3 p可以调节Myomaker的表达。
The fusion of myoblasts is an important step during skeletal muscle differentiation. A recent study in mice found that a transmembrane protein called Myomaker, which is specifically expressed in muscle, is critical for myoblast fusion. However, the cellular mechanism of its roles and the regulatory mechanism of its expression remain unclear. Chicken not only plays an important role in meat production but is also an ideal model organism for muscle development research. Here, we report that Myomaker is also essential for chicken myoblast fusion. Forced expression of Myomaker in chicken primary myoblasts promotes myoblast fusion, whereas knockdown of Myomaker by siRNA inhibits myoblast fusion. MYOD and MYOG, which belong to the family of myogenic regulatory factors, can bind to a conserved E-box located proximal to the Myomaker transcription start site and induce Myomaker transcription. Additionally, miR-140-3p can inhibit Myomaker expression and myoblast fusion, at least in part, by binding to the 3ʹ UTR of Myomaker in vitro. These findings confirm the essential roles of Myomaker in avian myoblast fusion and show that MYOD, MYOG and miR-140-3p can regulate Myomaker expression.