Antioxidant and anti proliferative activities of raspberries
Antioxidant and anti proliferative activities of raspberries
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DOI：
10.1021/jf0111209
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发表时间：
2002-05-08
影响因子：
6.1
通讯作者：
Liu, RH
中科院分区：
文献类型：
--
作者：
Liu, M;Li, XQ;Liu, RH
乌藨子富含酚类植物化学物质。为了研究乌藨子的健康益处，评估了四种新鲜乌藨子品种（Heritage，Kiwigold，Goldie和Anne）的总抗氧化和抗增殖活性。测定了四个树莓品种中每一个的酚类和黄酮类化合物的总量。传统树莓品种的总酚含量最高（512.7 +/- 4.7 mg/100 g乌藨子），其次是Kiwigold（451.1 +/- 4.5 mg/100 g乌藨子）、Goldie（427.5 +/- 7.5 mg/100 g乌藨子）和Anne（359.2 +/- 3.4 mg/100 g乌藨子）。同样，传统树莓品种含有最高的总黄酮测试品种的含量（103.4 +/- 2.0 mg/100 g乌藨子），其次是Kiwigold（87.3 +/- 1.8 mg/100 g乌藨子）、Goldie（84.2 +/- 1.8 mg/100 g乌藨子）和Anne（63.5 +/- 0.7 mg/100 g乌藨子）。树莓汁的颜色与树莓的总酚、类黄酮和花青素含量有很好的相关性。Heritage的a/B比值最高，果汁颜色最深，Anne品种的植物化学成分含量最低，颜色最浅。传统树莓品种的总抗氧化活性最高，其次是奇异果和戈尔迪，安妮树莓品种的抗氧化活性最低。乌藨子提取物对HepG（2）人肝癌细胞的增殖有明显的抑制作用，并呈剂量依赖性。相当于50 mg Goldie、Heritage和Kiwigold果实的提取物分别抑制这些细胞的增殖89.4 +/- 0.1、88 +/- 0.2和87.6 +/-1.0%。安妮具有最低的抗增殖活性的品种测量，但仍然表现出70.3 +/- 1.2%的显着抑制与提取物相当于50毫克的水果。乌藨子的抗氧化活性与乌藨子中酚类和黄酮类化合物的总量直接相关（p < 0.01）。同一树莓中酚类/黄酮类化合物的总量与其抗增殖活性之间没有相关性（p > 0.05）。
Raspberries are rich in phenolic phytochemicals. To study the health benefits of raspberries, four fresh raspberry varieties (Heritage, Kiwigold, Goldie, and Anne) were evaluated for total antioxidant and antiproliferative activities. The total amount of phenolics and flavonoids for each of the four raspberry varieties was determined. The Heritage raspberry variety had the highest total phenolic content (512.7 +/- 4.7 mg/100 g of raspberry) of the varieties measured followed by Kiwigold (451.1 +/- 4.5 mg/100 g of raspberry), Goldie (427.5 +/- 7.5 mg/100 g of raspberry), and Anne (359.2 +/- 3.4 mg/100 g of raspberry). Similarly, the Heritage raspberry variety contained the highest total flavonoids (103.4 +/- 2.0 mg/100 g of raspberry) of the varieties tested, followed by Kiwigold (87.3 +/- 1.8 mg/100 g of raspberry), Goldie (84.2 +/- 1.8 mg/100 g of raspberry), and Anne (63.5 +/- 0.7 mg/100 g of raspberry). The color of the raspberry juice correlated well to the total phenolic, flavonoid, and anthocyanin contents of the raspberry. Heritage had the highest a/b ratio and the darkest colored juice, and the Anne variety showed the lowest phytochemical content and the palest color. Heritage raspberry variety had the highest total antioxidant activity, followed by Kiwigold and Goldie, and the Anne raspberry variety had the lowest antioxidant activity of the varieties tested. The proliferation of HepG(2) human liver cancer cells was significantly inhibited in a dose-dependent manner after exposure to the raspberry extracts. The extract equivalent to 50 mg of Goldie, Heritage, and Kiwigold fruit inhibited the proliferation of those cells by 89.4 +/- 0.1, 88 +/- 0.2, and 87.6 +/- 1.0%, respectively. Anne had the lowest antiproliferative activity of the varieties measured but still exhibited a significant inhibition of 70.3 +/- 1.2% with an extract equivalent to 50 mg of fruit. The antioxidant activity of the raspberry was directly related to the total amount of phenolics and flavonoids found in the raspberry (p < 0.01). No relationship was found between antiproliferative activity and the total amount of phenolics/flavonoids found in the same raspberry (p > 0.05).