NPR2 is involved in FSH-mediated mouse oocyte meiotic resumption.
NPR2 is involved in FSH-mediated mouse oocyte meiotic resumption.
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DOI：
10.1186/s13048-016-0218-y
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发表时间：
2016-02-16
影响因子：
4
通讯作者：
Ma B
中科院分区：
文献类型：
--
作者：
Yang L;Wei Q;Li W;Xi Q;Zhao X;Ma B
以往的研究报道，卵泡刺激素(FSH)经常被添加到培养液中以诱导卵母细胞减数分裂恢复和成熟，并在体外成熟(IVM)过程中促进后续的胚胎发育。然而，潜在的机制仍不清楚。雌性小鼠腹腔注射8IU马绒毛膜促性腺激素后46~48h从卵巢采集卵丘-卵母细胞复合体(COCs)，并在不同的培养液中进行培养。QRT-PCR法检测EGF样因子和利钠肽受体2(NPR2)的mRNA表达。Western Blot分析丝裂原活化蛋白激酶3/1(MAPK3/1)的磷酸化水平。对卵母细胞进行了恢复减数分裂的形态分析。FSH刺激EGF样因子表达，MAPK3/1激活，NPR2基因表达减少，卵母细胞减数分裂恢复。此外，FSH诱导的NPR2减少和卵母细胞减数分裂恢复是通过MAPK3/1信号通路发生的，该通路由表皮生长因子受体(EGFR)激活。NPR2参与FSH介导的卵母细胞减数分裂恢复，这一过程与EGFR和MAPK3/1信号通路有关。
Previous studies have reported that follicle-stimulating hormone (FSH) is often added to culture media to induce oocyte meiotic resumption and maturation and to improve subsequent embryonic development during in vitro maturation (IVM). However, the underlying mechanisms remain unclear. Cumulus-oocyte complexes (COCs) were collected from ovaries 46–48 h after the female mice were intraperitoneally injected with 8 IU equine chorionic gonadotropin (eCG) and then the COCs were cultured in different medium. qRT-PCR analysis was used to assess mRNA expression of EGF-like factors and natriuretic peptide receptor 2 (NPR2). Western Blot analysis was used to assess phosphorylation of mitogen-activated protein kinase 3/1 (MAPK3/1). The oocytes were morphologically assessed for meiotic resumption. FSH stimulated the expression of EGF-like factors, the activation of MAPK3/1, a decrease in NPR2 mRNA and oocyte meiotic resumption. Moreover, the FSH-induced decrease in NPR2 and oocyte meiotic resumption occurred via the MAPK3/1 singling pathway, which was activated by the epidermal growth factor receptor (EGFR) pathway. NPR2 is involved in FSH-mediated oocyte meiotic resumption, and this process is associated with the EGFR and MAPK3/1 signaling pathways.