Involvement of aquaporins in colorectal carcinogenesis
Involvement of aquaporins in colorectal carcinogenesis
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DOI：
10.1038/sj.onc.1206762
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发表时间：
2003-10-02
期刊：
影响因子：
8
通讯作者：
Mao, L
中科院分区：
文献类型：
--
作者：
Moon, C;Soria, JC;Mao, L
水通道蛋白（AQP）在控制水渗透性中是重要的。由于AQP 1是一种血清应答基因，我们推测AQP的表达可能与人类癌症的发生有关。通过逆转录-聚合酶链反应分析，AQP 1，3和5的表达被发现在7个结肠和结直肠癌细胞系。Western印迹分析证实了它们在这些细胞系中的四个中的表达。原位杂交表明，在结直肠癌的发生过程中，水通道蛋白1和5的表达诱导在早期疾病（早期异型增生），并保持通过结肠癌发展的晚期阶段。AQP 1和5的表达即使在肝转移灶中也保持不变。这些发现表明，在肿瘤细胞中发现了几种AQP的表达，并且与结直肠癌发展的早期阶段相关。这些新的观察表明，多个AQP表达可能是有利的肿瘤发生，这可能会导致更好地了解结直肠癌的发生。
Aquaporins (AQPs) are important in controlling water permeability. As AQP1 is known as a serum-responsive gene, we hypothesized that AQP expression may be involved in the development of human cancer. By reverse transcriptase-polymerase chain reaction analysis, expression of AQPs 1, 3, and 5 was found in seven colon and colorectal cancer cell lines. Western blot analysis confirmed their expression in four of these cell lines. In situ hybridization demonstrated that during colorectal carcinogenesis, the expression of AQPs 1 and 5 was induced in early-stage disease ( early dysplasia) and maintained through the late stages of colon cancer development. Expression of AQPs 1 and 5 was maintained even in metastatic lesions in the liver. These findings demonstrate that the expression of several AQPs is found in tumor cells and is associated with an early stage of colorectal cancer development. These novel observations suggest that multiple AQP expression may be advantageous to tumorigenesis, which may lead to a better understanding of colorectal carcinogenesis.