Regulatory function of Arabidopsis lipid transfer protein 1 (LTP1) in ethylene response and signaling
Regulatory function of Arabidopsis lipid transfer protein 1 (LTP1) in ethylene response and signaling
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拟南芥脂质转移蛋白 1 (LTP1) 在乙烯反应和信号传导中的调节功能
DOI：
10.1007/s11103-016-0482-7
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发表时间：
2016
影响因子：
5.1
通讯作者：
Dong Chun-Hai
中科院分区：
文献类型：
--
作者：
Wang Honglin;Sun Yue;Chang Jianhong;Zheng Fangfang;Pei Haixia;Yi Yanjun;Chang Caren;Dong Chun-Hai
乙烯作为一种气态植物激素，直接参与植物生长发育的各种过程。关于乙烯受体和乙烯信号转导途径中的调节因子，人们已经知道了很多。在拟南芥中，逆转为乙烯SENSITIVITY1(RTE1)可以与乙烯受体乙烯反应1(ETR1)相互作用并正向调节。在这项研究中，我们报道了一个与RTE1相互作用的蛋白，一个功能未知的拟南芥脂转移蛋白1(LTP1)的鉴定和性质。通过双分子荧光互补，在PLANTA中验证了LTP1与RTE1之间的直接分子相互作用。对转基因植物中LTP1-GFP融合的分析和质壁分离实验表明，LTP1定位于细胞质中。对乙烯反应的分析表明，LTP1基因敲除对1-氨基环丙烷羧酸(ACC)高度敏感，而LTP1过表达则表现出不敏感。对双突变体setr1-2ltp1和rte1-3ltp1的分析表明，LTP1通过与RTE1的分子结合而在乙烯受体信号转导中发挥调节作用。本研究揭示了拟南芥LTP1在调节乙烯反应和信号转导中的一个新功能。
Ethylene as a gaseous plant hormone is directly involved in various processes during plant growth and development. Much is known regarding the ethylene receptors and regulatory factors in the ethylene signal transduction pathway. InArabidopsis thaliana, REVERSION-TO-ETHYLENE SENSITIVITY1 (RTE1) can interact with and positively regulates the ethylene receptor ETHYLENE RESPONSE1 (ETR1). In this study we report the identification and characterization of an RTE1-interacting protein, a putative Arabidopsis lipid transfer protein 1 (LTP1) of unknown function. Through bimolecular fluorescence complementation, a direct molecular interaction between LTP1 and RTE1 was verified in planta. Analysis of an LTP1-GFP fusion in transgenic plants and plasmolysis experiments revealed that LTP1 is localized to the cytoplasm. Analysis of ethylene responses showed that theltp1knockout is hypersensitive to 1-aminocyclopropanecarboxylic acid (ACC), whileLTP1overexpression confers insensitivity. Analysis of double mutantsetr1-2 ltp1andrte1-3 ltp1demonstrates a regulatory function of LTP1 in ethylene receptor signaling through the molecular association with RTE1. This study uncovers a novel function of Arabidopsis LTP1 in the regulation of ethylene response and signaling.