Selective transcription and modulation of resting T cell activity by preintegrated HIV DNA
Selective transcription and modulation of resting T cell activity by preintegrated HIV DNA
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DOI：
10.1126/science.1061548
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发表时间：
2001-08-24
期刊：
影响因子：
56.9
通讯作者：
Marsh, JW
中科院分区：
文献类型：
--
作者：
Wu, YT;Marsh, JW
大多数外周T细胞的静止特性有效地限制了人类免疫缺陷病毒（HIV）的复制，特别是病毒与宿主染色质的整合。两种HIV蛋白，Nef和Tat，可以增加T细胞的活性，但是病毒基因表达的整合要求会阻止这些蛋白在静息细胞中发挥作用。在这里，我们报道了HIV感染导致nef和这些基因在整合之前的选择性转录。静止细胞中的这种整合前转录导致T细胞活化和病毒复制增加。
The quiescent nature of most peripheral T cells poses an effective limitation to human immunodeficiency virus (HIV) replication and, in particular, to viral integration into the host chromatin. Two HIV proteins, Nef and Tat, increase T cell activity, but a requirement of integration for viral gene expression would preclude a rote for these proteins in resting cells. Here, we report that HIV infection leads to selective transcription of the nef and tat genes before integration. This preintegration transcription in quiescent cells leads to increased T cell activation and viral replication.