Molecular adaptations of neuromuscular disease-associated proteins in response to eccentric exercise in human skeletal muscle
Molecular adaptations of neuromuscular disease-associated proteins in response to eccentric exercise in human skeletal muscle
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DOI：
10.1113/jphysiol.2002.018689
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发表时间：
2002-08-15
影响因子：
5.5
通讯作者：
Denis, C
中科院分区：
文献类型：
--
作者：
Féasson, L;Stockholm, D;Denis, C
偏心性肌肉收缩诱导肌肉损伤和重塑的分子事件在很大程度上仍然未知。我们评估了离心运动是否调节蛋白酶（钙蛋白酶1、2和3、蛋白酶体、组织蛋白酶B+L）、肌肉结构蛋白（α-肌聚糖和结蛋白）的表达以及热休克蛋白Hsp 27和α B-晶状体蛋白的表达。12名健康男性志愿者的股外侧肌活检前，后立即，1和14天后，30分钟的下坡跑步机跑步锻炼。离心运动引起的肌肉损伤的肌肉疼痛和无力，肌酸激酶血清活性，肌红蛋白血症和肌肉活检的超微结构分析证明。钙蛋白酶3 mRNA水平在运动后立即下降，而钙蛋白酶2 mRNA水平在第1天增加。到第14天，两种mRNA水平均恢复至对照值。相比之下，组织蛋白酶B+L和蛋白酶体酶活性在第14天增加。α-肌聚糖蛋白水平在运动后立即和第1天降低，而结蛋白水平在第14天达到峰值。α B-晶状体蛋白和Hsp 27蛋白水平在第1天和第14天增加。我们的研究结果表明，钙蛋白酶2和3 mRNA水平的差异表达可能是重要的运动诱导的肌肉损伤的过程中，而α-肌聚糖，结蛋白，α B-晶体蛋白和热休克蛋白27的表达可能基本上参与了随后的肌原纤维结构的重塑。这种重塑反应可以限制肌肉在随后的机械应力下的损伤程度。
The molecular events by which eccentric muscle contractions induce muscle damage and remodelling remain largely unknown. We assessed whether eccentric exercise modulates the expression of proteinases (calpains 1, 2 and 3, proteasome, cathepsin B+L), muscle structural proteins (alpha-sarcoglycan and desmin), and the expression of the heat shock proteins Hsp27 and alphaB-crystallin. Vastus lateralis muscle biopsies from twelve healthy male volunteers were obtained before, immediately after, and 1 and 14 days after a 30 min downhill treadmill running exercise. Eccentric exercise induced muscle damage as evidenced by the analysis of muscle pain and weakness, creatine kinase serum activity, myoglobinaemia and ultrastructural analysis of muscle biopsies. The calpain 3 mRNA level was decreased immediately after exercise whereas calpain 2 mRNA level was increased at day 1. Both mRNA levels returned to control values by day 14. By contrast, cathepsin B+L and proteasome enzyme activities were increased at day 14. The a-sarcoglycan protein level was decreased immediately after exercise and at day 1, whereas the desmin level peaked at day 14. alphaB-crystallin and Hsp27 protein levels were increased at days 1 and 14. Our results suggest that the differential expression of calpain 2 and 3 mRNA levels may be important in the process of exercise-induced muscle damage, whereas expression of alpha-sarcoglycan, desmin, alphaB-crystallin and Hsp27 may be essentially involved in the subsequent remodelling of myofibrillar structure. This remodelling response may limit the extent of muscle damage upon a subsequent mechanical stress.