Adult rat mesenchymal stem cells differentiate into neuronal-like phenotype and express a variety of neuro-regulatory molecules in vitro
Adult rat mesenchymal stem cells differentiate into neuronal-like phenotype and express a variety of neuro-regulatory molecules in vitro
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成年大鼠间充质干细胞在体外分化为神经元样表型并表达多种神经调节分子
DOI：
10.1016/j.neures.2009.09.1711
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发表时间：
2010-01-01
影响因子：
2.9
通讯作者：
Xu, Jianguang
中科院分区：
文献类型：
--
作者：
Jiang, Junjian;Lv, Zhongwei;Xu, Jianguang
骨髓基质干细胞（MSCs），通常分化为间充质衍生物，最近报道转分化为神经元。本文旨在重新评价MSCs向神经元转分化的现象，并比较rMSCs及其衍生的神经元样表型中神经营养因子的表达水平。我们将rMSCs置于2-巯基乙醇和2%二甲基亚砜中5 h。然后将细胞转移到由DMEM + 10%FBS、10 μ g/L碱性成纤维细胞生长因子、10 μ g/L人表皮生长因子、1 mmol二丁酰环磷酸腺苷和0.5mmol异丁基甲基黄嘌呤组成的神经元诱导培养基中7天和14天。本研究表明，rMSCs中BDNF、NGF、NT 3、CNTF和GDNF的表达水平明显高于神经元样表型，尤其是CNTF。这些神经营养因子的表达水平在持续诱导后没有显著变化。我们认为rMSCs在一定条件下可以转分化为神经元样表型。未诱导的rMSCs具有神经营养因子的动态表达谱，并且可以作为比转分化的rMSCs更好的移植治疗工具。(C)2009年爱思唯尔爱尔兰有限公司和日本神经科学学会。All rights reserved.
Bone marrow stromal stem cells (MSCs), which normally differentiate into mesenchymal derivatives, have recently reported to trans-differentiate into neurons. However, the findings from different groups and interpretations have been challenged.The purpose of this paper is to re-evaluate the phenomenon of neuronal trans-differentiation of MSCs and compare the expression levels of neurotrophins in rMSCs and neuronal-like phenotypes derived from rMSCs. We put rMSCs in 2-mercaptoethanol and 2% dimethylsulfoxide for 5 h. Then, the cells were transferred to neuronal induction media composed of DMEM + 10%FBS, 10 ug/L basic fibroblast growth factor, 10 ug/L human epidermal growth factor, I mmol dibutyryl cyclicn AMP and 0.5 mmol isobutylmethylxanthine for 7 days and 14 days. The study demonstrated that the level of BDNF, NGF, NT3, CNTF and GDNF of rMSCs is remarkably higher in rMSCs than the neuronal-like phenotypes, especially CNTF. The expression level of these neurotrophins did not change significantly after enduring induction. We believed that rMSCs can trans-differentiate into neuronal-like phenotype under certain conditions. The non-induced rMSCs has a dynamic expression profile of neurotrophins and may serves as a better tool than the trans-differentiated rMSCs for transplant therapy. (C) 2009 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.