Cell migration and invasion assays as tools for drug discovery.
Cell migration and invasion assays as tools for drug discovery.
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DOI：
10.3390/pharmaceutics3010107
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发表时间：
2011-03-11
期刊：
影响因子：
5.4
通讯作者：
Herber RL
中科院分区：
文献类型：
--
作者：
Hulkower KI;Herber RL
细胞迁移和侵袭是为干预关键生理和病理现象如伤口愈合和癌症转移提供丰富靶点的过程。随着高通量和高含量成像系统的出现，在测试范例中已经出现了朝向更早地使用生理相关的基于细胞的测定的运动。这允许在药物发现筛选过程中更有效地鉴定先导化合物并更快地识别不良反应。本文将综述几种研究细胞运动性的主要方法：划痕法、跨膜法、微流控装置和细胞排阻区法。
Cell migration and invasion are processes that offer rich targets for intervention in key physiologic and pathologic phenomena such as wound healing and cancer metastasis. With the advent of high-throughput and high content imaging systems, there has been a movement towards the use of physiologically relevant cell-based assays earlier in the testing paradigm. This allows more effective identification of lead compounds and recognition of undesirable effects sooner in the drug discovery screening process. This article will review the effective use of several principle formats for studying cell motility: scratch assays, transmembrane assays, microfluidic devices and cell exclusion zone assays.