A small nuclear ribonucleoprotein is required for splicing of adenoviral early RNA sequences.
A small nuclear ribonucleoprotein is required for splicing of adenoviral early RNA sequences.
复制标题
腺病毒早期 RNA 序列的剪接需要一种小的核核糖核蛋白。
DOI：
10.1073/pnas.78.3.1371
复制
发表时间：
1981
影响因子：
11.1
通讯作者：
Flint,SJ
中科院分区：
文献类型：
--
作者：
Yang,VW;Lerner,MR;Steitz,JA;Flint,SJ
通过变性聚丙烯酰胺细胞电泳和核酸酶S1测定，研究了在2型腺病毒感染早期分离的细胞核中合成的病毒RNA种类的大小和结构。腺病毒基因组中早期区域1和2的体外转录的主要产物是经过加工的RNA分子，这些分子似乎在分离的细胞核中正确拼接。当细胞核与来自系统性红斑狼疮患者的抗体预孵育时，腺病毒RNA分子的剪接被抑制，所述抗体免疫沉淀小的核核糖核蛋白颗粒。这些抗体的特异性表明，含有U1 RNA的核糖核蛋白颗粒是我们所研究的腺病毒RNA序列剪接所必需的。
The size and structure of viral RNA species synthesized in nuclei isolated during the early phase of productive infection by adenovirus type 2 have been examined by electrophoresis in denaturing polyacrylamide cells and the nuclease S1 assay. The major products of transcription in vitro of early regions 1 and 2 in the adenoviral genome are processed RNA molecules that appear to be correctly spliced in isolated nuclei. Splicing of adenoviral RNA molecules is inhibited when nuclei are preincubated with antibodies from systemic lupus erythematosus patients that immunoprecipitate small nuclear ribonucleoprotein particles. The specificity of these antibodies suggests that ribonucleoprotein particles containing U1 RNA are required for splicing of the adenoviral RNA sequences we have examined.