Superresolution imaging of dynamic MreB filaments in B. subtilis--a multiple-motor-driven transport?
Superresolution imaging of dynamic MreB filaments in B. subtilis--a multiple-motor-driven transport?
复制标题
枯草芽孢杆菌中动态 MreB 丝的超分辨率成像——多电机驱动的运输？
DOI：
10.1016/j.bpj.2013.07.038
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发表时间：
2013
影响因子：
3.4
通讯作者：
A. Rohrbach
中科院分区：
文献类型：
--
作者：
v. Olshausen P;H. J. Defeu Soufo;K. Wicker;R. Heintzmann;P. L. Graumann;A. Rohrbach
The cytoskeletal protein MreB is an essential component of the bacterial cell-shape generation system. Using a superresolution variant of total internal reflection microscopy with structured illumination, as well as three-dimensional stacks of deconvolved epifluorescence microscopy, we found that inside livingBacillus subtiliscells, MreB forms filamentous structures of variable lengths, typically not longer than 1μm. These filaments move along their orientation and mainly perpendicular to the long bacterial axis, revealing a maximal velocity at an intermediate length and a decreasing velocity with increasing filament length. Filaments move along straight trajectories but can reverse or alter their direction of propagation. Based on our measurements, we provide a mechanistic model that is consistent with all observations. In this model, MreB filaments mechanically couple several motors that putatively synthesize the cell wall, whereas the filaments' traces mirror the trajectories of the motors. On the basis of our mechanistic model, we developed a mathematical model that can explain the nonlinear velocity length dependence. We deduce that the coupling of cell wall synthesis motors determines the MreB filament transport velocity, and the filament mechanically controls a concerted synthesis of parallel peptidoglycan strands to improve cell wall stability.
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影响因子：
7.7
作者：
Soufo, HJD;Graumann, PL
通讯作者：
Graumann, PL
影响因子：
3.6
作者：
Soufo, Herve Joel Defeu;Graumann, Peter L.
通讯作者：
Graumann, Peter L.
影响因子：
3.4
作者：
Gustafsson, Mats G. L.;Shao, Lin;Sedat, John W.
通讯作者：
Sedat, John W.
影响因子：
4.8
作者：
Esue, O;Cordero, M;Tseng, Y
通讯作者：
Tseng, Y
影响因子：
--
作者：
Defeu Soufo HJ;Graumann PL
通讯作者：
Graumann PL