Autotaxin Is Required for the Cranial Neural Tube Closure and Establishment of the Midbrain-Hindbrain Boundary During Mouse Development
Autotaxin Is Required for the Cranial Neural Tube Closure and Establishment of the Midbrain-Hindbrain Boundary During Mouse Development
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DOI：
10.1002/dvdy.22543
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发表时间：
2011-02-01
影响因子：
2.5
通讯作者：
Ohuchi, Hideyo
中科院分区：
文献类型：
--
作者：
Koike, Seiichi;Yutoh, Yoshifumi;Ohuchi, Hideyo
Autotaxin （ATX）是一种在胚胎和成人神经组织中表达的产生溶血磷脂的外酶。我们之前的研究表明，ATX在神经组织中心、前脑突和中脑后脑边界（MHB）表达。为了阐明ATX在神经发育中的作用，我们研究了ATX缺陷小鼠的神经表型。神经标记基因的表达分析显示，早在头襞晚期，ATX突变胚胎的吻侧前脑的横向扩张就减少了，MHB的建立也受到了损害。此外，ATX突变胚胎不能完成颅神经管闭合。这些结果表明，ATX对颅神经的形成和MHB的建立至关重要。科学进展，2011(4):413-421。(C) 2011 Wiley-Liss, Inc。
Autotaxin (ATX) is a lysophospholipid-generating exoenzyme expressed in embryonic and adult neural tissues. We previously showed that ATX is expressed in the neural organizing centers, anterior head process, and midbrain-hindbrain boundary (MHB). To elucidate the role of ATX during neural development, here we examined the neural phenotypes of ATX-deficient mice. Expression analysis of neural marker genes revealed that lateral expansion of the rostral forebrain is reduced and establishment of the MHB is compromised as early as the late headfold stage in ATX mutant embryos. Moreover, ATX mutant embryos fail to complete cranial neural tube closure. These results indicate that ATX is essential for cranial neurulation and MHB establishment. Developmental Dynamics 240:413-421, 2011. (C) 2011 Wiley-Liss, Inc.