Spontaneous immunoglobulin‐producing capacity of cultures from lupus patients and normal donors following depletion of cells expressing CD19 or CD38
Spontaneous immunoglobulin‐producing capacity of cultures from lupus patients and normal donors following depletion of cells expressing CD19 or CD38
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DOI：
10.1046/j.1365-2249.1998.00533.x
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发表时间：
1998-03
影响因子：
4.6
通讯作者：
Bourne;Zukowska‐Cooper;Salaman;Seifert;Isenberg
中科院分区：
文献类型：
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作者：
Bourne;Zukowska‐Cooper;Salaman;Seifert;Isenberg
系统性红斑狼疮 (SLE) 患者血液中自发分泌 IgG 或 IgM (ISC) 的细胞含量很高。通过使用磁珠技术，根据 CD19 或 CD38 的表达对来自此类患者和健康供体的单核细胞进行分级，然后将细胞级分在不添加丝裂原/抗原的情况下培养 5/6 天。测定上清液 IgG 和 IgM，此外，在 CD38 实验中，在培养之前和之后对 ISC 进行计数。两个供体组的未分级细胞 (UFC) 的大部分免疫球蛋白生成能力在 CD19- 部分中得到恢复，而 CD19+ 细胞没有产生免疫球蛋白，这表明 ISC 不表达 CD19。相比之下，CD38 分级分离导致几乎所有 ISC 都转移到 CD38+ 级分，其产生的免疫球蛋白水平接近 UFC 的 50%。在 CD38− 细胞培养中，IgG 和 IgM ISC 的数量有所增加，这在对照组中尤其引人注目，其数量远远超过 UFC 中的数量。并非所有这些新的 ISC 都变成 CD38+，但 SLE 患者的成熟过程更加有效。讨论了 CD38− 群体中自发反应的可能性是由于 CD38+ 自然杀伤 (NK) 细胞的去除。去除预培养中存在的 ISC 是研究疾病中 ISC 生成的有用的第一步。
Cells spontaneously secreting IgG or IgM (ISC) are present at a high level in the blood of patients with systemic lupus erythematosus (SLE). By use of magnetic‐bead techniques, mononuclear cells from such patients and healthy donors were fractionated according to expression of CD19 or CD38 and the cell fractions were then cultured in the absence of added mitogen/antigen for 5/6 days. Supernatant IgG and IgM were determined and, in addition, in the CD38 experiments ISC were enumerated both before and after culture. Much of the immunoglobulin‐producing capacity of unfractionated cells (UFC) from both donor groups was recovered in the CD19− fraction, and no immunoglobulin was produced by CD19+ cells suggesting, unexpectedly, that ISC were not expressing CD19. By contrast, CD38 fractionation resulted in nearly all ISC passing to the CD38+ fraction which produced levels of immunoglobulin approaching 50% that of UFC. On culture of CD38− cells there was a build up in the number of IgG and IgM ISC, this being particularly striking in the controls with numbers well in excess of those in UFC. Not all these new ISC became CD38+, but the maturation process was more efficient in the SLE patients. The possibility is discussed that the spontaneous response in the CD38− populations is due to removal of CD38+ natural killer (NK) cells. Removal of ISC that are present preculture is a helpful initial step in studying ISC generation in the disease.