Studies of the Mobile Phase in Gel Permeation Chromatography for Analysis of Pesticide Residues
Studies of the Mobile Phase in Gel Permeation Chromatography for Analysis of Pesticide Residues
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凝胶渗透色谱农药残留分析流动相的研究
DOI：
10.3358/shokueishi.38.16
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
S. Hori
中科院分区：
文献类型：
--
作者：
M. Okihashi;H. Obana;S. Hori
为了寻找一种不使用二氯甲烷的凝胶渗透色谱（GPC）移动的相，我们用GPC在两个柱上对玉米油、菠菜色素、辣椒色素、氟胺氰菊酯和氨基硫脲进行了分离。本研究中使用的溶剂混合物为乙酸乙酯-环己烷（1：1）、乙酸乙酯-环己烷（3：7）和丙酮-环己烷（3：7）。当农药馏分被确定为可能的最大收集从氟胺氰菊酯，甲胺磷，玉米油和辣椒红色素几乎被排除在农药馏分，使用富含环己烷的洗脱液。用丙酮-环己烷（3：7）作洗脱剂，菠菜色素的去除率为71%。结果表明，用于残留农药分析的合适混合物是丙酮-环己烷（3：7）和乙酸乙酯-环己烷（3：7），这取决于柱的选择。
In order to find a suitable mobile phase for gel permeation chromatography (GPC) without using dichloromethane, we have fractionated corn oil, spinach color, paprika color, fluvalinate and chinomethionat by GPC on two columns. The solvent mixtures used in this study were ethyl acetate-cyclohexane (1: 1), ethyl acetate-cyclohexane (3: 7) and acetone-cyclohexane (3: 7). When the pesticide fraction was identified as the possible maximum collection from fluvalinate to chinomethionat, corn oil and paprika color were practically excluded from the pesticide fraction, using a cyclohexane-rich eluent. Spinach color was 71% eliminated from the pesticide fraction with acetone-cyclohexane (3: 7) as the eluent. The results suggest that suitable mixtures for residual pesticide analysis would be acetone-cyclohexane (3: 7) and ethyl acetate-cyclohexane (3: 7), depending upon the column selection.