WHOLE-GENOME RANDOM SEQUENCING AND ASSEMBLY OF HAEMOPHILUS-INFLUENZAE RD
WHOLE-GENOME RANDOM SEQUENCING AND ASSEMBLY OF HAEMOPHILUS-INFLUENZAE RD
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DOI：
10.1126/science.7542800
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发表时间：
1995-07-28
期刊：
影响因子：
56.9
通讯作者：
VENTER, JC
中科院分区：
文献类型：
--
作者：
FLEISCHMANN, RD;ADAMS, MD;VENTER, JC
已经采用了基于整个染色体的未选择的DNA的测序和组装基因组分析的方法，以获取来自流感嗜血杆菌嗜血杆菌细菌的基因组的完整核苷酸序列（1,830,137碱基对）。这种方法消除了对初始映射工作的需求，因此适用于基因组图不可用的大量微生物物种。 H. h. h. h. pranp. rd基因组序列（基因组序列数据库登录号L42023）代表了自由生物生物体唯一的完整基因组序列。
An approach for genome analysis based on sequencing and assembly of unselected pieces of DNA from the whole chromosome has been applied to obtain the complete nucleotide sequence (1,830,137 base pairs) of the genome from the bacterium Haemophilus influenzae Rd. This approach eliminates the need for initial mapping efforts and is therefore applicable to the vast array of microbial species for which genome maps are unavailable. The H. influenzae Rd genome sequence (Genome Sequence DataBase accession number L42023) represents the only complete genome sequence from a free-living organism.