Biodegradation of triphenylmethane dye Malachite Green by Sphingomonas paucimobilis
Biodegradation of triphenylmethane dye Malachite Green by Sphingomonas paucimobilis
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DOI：
10.1007/s11274-008-9941-x
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发表时间：
2009-04-01
影响因子：
4.1
通讯作者：
Bakhrouf, Amina
中科院分区：
文献类型：
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作者：
Ayed, Lamia;Chaieb, Kamel;Bakhrouf, Amina
三苯甲烷染料是最重要的一类合成染料，在纺织工业中广泛用于染色棉、毛、丝、尼龙等。它们通常被认为是难以被生物降解的异种化合物。从突尼斯ksarhelal纺织工业污染场地土壤样品中分离到少动鞘氨单胞菌（Sphingomonas haucimobilis），在pH为9、温度为25℃的摇动条件下，对孔雀石绿（50 MG /l）染料进行脱色，脱色时间为4 h。研究了接种量、染料浓度、温度和溶液初始pH值等因素的影响。从批量实验中获得的结果揭示了被测细菌去除染料的能力。对脱色前后样品的紫外可见光谱和红外光谱分析证实了该菌株对MG脱色的能力。此外，植物毒性研究表明，S. paucimobilis可将MG降解为无毒产物。
Triphenylmethane dyes belong to the most important group of synthetic colorants and are used extensively in the textile industries for dying cotton, wool, silk, nylon, etc. They are generally considered as the xenobiotic compounds, which are very recalcitrant to biodegradation. Sphingomonas paucimobilis, was isolated from the soil sample collected from contaminated sites of textile industry located in KsarHellal, Tunisia, and it was able to decolorize Malachite Green (MG) dye (50 mg/l) within 4 h under shaking condition (pH 9 and temperature 25A degrees C). The effect of inoculum size, dye concentration, temperature and initial pH of the solution were studied. The results obtained from the batch experiments revealed the ability of the tested bacteria to remove dye. UV-Vis spectroscopy and FTIR analysis of samples before and after decolorization confirmed the ability of the tested strain to decolorize MG. In addition, the phytotoxicity study revealed the degradation of MG into non-toxic product by S. paucimobilis.