The expression and tyrosine phosphorylation of E-cadherin/catenin adhesion complex, and focal adhesion kinase in invasive cervical carcinomas
The expression and tyrosine phosphorylation of E-cadherin/catenin adhesion complex, and focal adhesion kinase in invasive cervical carcinomas
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DOI：
10.1046/j.1525-1438.2003.13396.x
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发表时间：
2003-09-01
影响因子：
4.8
通讯作者：
Kim, SC
中科院分区：
文献类型：
--
作者：
Moon, HS;Park, WI;Kim, SC
本研究旨在证实E-cadherin/catenin黏附复合物的表达和磷酸化状态与宫颈癌发生和宫颈癌侵袭有关的假设，并探讨局灶黏附激酶（focal adhesion kinase， FAK）的表达和酪氨酸磷酸化及其与E-cadherin/catenin黏附复合物的关系。应用western blot方法研究了26例宫颈癌、9例正常宫颈和5例宫颈原位癌组织中E-cadherin、α -和β -catenin及FAK的表达。免疫沉淀法检测α -和β -连环蛋白和FAK的酪氨酸磷酸化。α -和β -catenin及E-cadherin在宫颈癌中的表达降低，α -和β -catenin酪氨酸磷酸化水平在宫颈癌中高于正常宫颈和宫颈原位癌。在宫颈癌中，FAK的酪氨酸磷酸化水平升高，但FAK的表达差异无统计学意义。此外，α -和β -连环蛋白与FAK共免疫沉淀。我们得出结论，E-cadherin/catenin蛋白的缺失和E-cadherin/catenin的酪氨酸磷酸化参与了宫颈癌的发生和癌症的侵袭。黏附激酶酪氨酸磷酸化也与宫颈癌侵袭有关。E-cadherin/catenin复合物与FAK可能在功能和结构上有关联。
The present study aimed to confirm the hypothesis that the expression and phosphorylation status of the E-cadherin/catenin adhesion complex is related to cervical carcinogenesis and cervical cancer invasion, and to investigate the expression and the tyrosine phosphorylation of focal adhesion kinase (FAK) and its relation with E-cadherin/catenin adhesion complex. The expression of E-cadherin, alpha- and beta-catenin, and FAK were studied by a western blot analysis with 26 cervical carcinomas, nine normal cervices, and five carcinomas in situ of cervix. The tyrosine phosphorylation of alpha- and beta-catenin and FAK were examined by an immunoprecipitation. The expressions of alpha- and beta-catenin and E-cadherin were reduced in cervical carcinoma, and the tyrosine phosphorylation of alpha- and beta-catenin in cervical carcinoma was higher than in normal cervix and carcinoma in situ of cervix. Tyrosine phosphorylation of FAK was elevated in cervical carcinoma although the expression of FAK was not significantly different. Moreover, alpha- and beta-catenin were coimmunoprecipitated with FAK. We conclude that the loss of E-cadherin/catenin proteins and the tyrosine phosphorylation of E-cadherin/catenin are involved in cervical carcinogenesis and cancer invasion. Tyrosine phosphorylation of focal adhesion kinase is also related to the cervical cancer invasion. The E-cadherin/catenin complex and FAK may be related functionally and structurally.