Fitness cost in field and laboratory Aedes aegypti populations associated with resistance to the insecticide temephos.
Fitness cost in field and laboratory Aedes aegypti populations associated with resistance to the insecticide temephos.
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DOI：
10.1186/s13071-015-1276-5
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发表时间：
2015-12-30
影响因子：
3.2
通讯作者：
Ayres CF
中科院分区：
文献类型：
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作者：
Diniz DF;de Melo-Santos MA;Santos EM;Beserra EB;Helvecio E;de Carvalho-Leandro D;dos Santos BS;de Menezes Lima VL;Ayres CF
在巴西国家登革热控制计划的背景下，化学杀虫剂的持续使用对埃及伊蚊的自然种群产生了很大的选择压力，导致它们在野外对这些化合物产生抗药性。健身成本被描述为抵抗力的适应性后果。这项研究评估了埃及伊蚊菌株的生物学和繁殖性能，以及一个对泰米福斯(用于控制蚊子的主要杀幼虫剂)具有抗性的田间种群。对来自巴西伯南布哥州阿科瓦德市的高抗虫率(RR = 226.6)的田间抗性种群和来自同一来源(伯南布哥州阿拉里皮纳市)的三个相同的实验室菌株进行了比较试验：RECR(RR = 283.6)；RECRNEX(RR = 250.5)，处于抗性恢复过程中的菌株；以及作为实验对照的反向敏感菌株RECREV(RR = 2.32)。我们的研究表明，连续五代暴露于temephos没有施加选择性压力，导致抗药性比率和解毒酶反应的离散降低。在抗性品系和田间种群中，19个生物学参数中的大部分都受到了损害。对生育力生命表的分析证实，抗性个体存在生殖劣势。同样，抗性雌虫的寿命、体型和总能量来源也较低，但田间雌虫(Aroverde)的后两个参数除外。相比之下，性别比和胚胎存活率在所有被评估的品系或种群中都没有受到干扰，无论它们对泰米福斯的抗性状态如何。抗性个体的繁殖潜力和生存能力都受到了影响。影响最大的参数是幼虫发育时间、繁殖力、净繁殖率和世代倍增时间。所研究的自然种群和实验室菌株的这些适应成本可能与维持对泰米福斯的抗药性的代谢机制有关。我们的结果表明，尽管付出了这些代价，但高度抗硫磷的种群可以弥补这些损失，并成功地克服基于使用化学杀虫剂的控制行动。
The continued use of chemical insecticides in the context of the National Program of Dengue Control in Brazil has generated a high selective pressure on the natural populations of Aedes aegypti, leading to their resistance to these compounds in the field. Fitness costs have been described as adaptive consequences of resistance. This study evaluated the biological and reproductive performance of A. aegypti strains and a field population resistant to temephos, the main larvicide used for controlling mosquitoes. Comparative tests were performed with a resistant field population from the municipality of Arcoverde, Pernambuco State, Brazil, with a high rate of temephos resistance (RR = 226.6) and three isogenetic laboratory strains from the same origin (Araripina municipality, Pernambuco): RecR (RR = 283.6); RecRNEx (RR = 250.5), a strain under a process of resistance reversion; and RecRev (RR = 2.32), a reversed susceptible strain used as an experimental control. Our study revealed that the absence of selective pressure imposed by exposure to temephos, for five consecutive generations, led to a discrete reduction of the resistance ratio and the response of the detoxifying enzymes. Most of the 19 biological parameters were impaired in the resistant strains and field population. The analysis of the fertility life table confirmed the presence of reproductive disadvantages for the resistant individuals. Similarly, the longevity, body size, and total energetic resources were also lower for the resistant females, except for the last two parameters in the field females (Arcoverde). In contrast, the sex ratio and embryonic viability suffered no interference in all strains or population evaluated, regardless of their status of resistance to temephos. The reproductive potential and survival of the resistant individuals were compromised. The parameters most affected were the larval development time, fecundity, net reproduction rate, and the generational doubling time. These fitness costs in the natural population and laboratory strains investigated are likely associated with maintaining the metabolic mechanism of resistance to temephos. Our results show that despite these costs, the highly temephos resistant populations can compensate for these losses and successfully overcome the control actions that are based on the use of chemical insecticides.