Involvement of brain catalase activity in the acquisition of ethanol-induced conditioned place preference
Involvement of brain catalase activity in the acquisition of ethanol-induced conditioned place preference
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DOI：
10.1016/j.physbeh.2007.11.026
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发表时间：
2008-03-18
影响因子：
2.9
通讯作者：
Aragon, Carlos M. G.
中科院分区：
文献类型：
--
作者：
Font, Laura;Miquel, Marta;Aragon, Carlos M. G.
有人认为，乙醇产生的一些行为效应是由其第一代谢产物乙醛介导的。本研究提出了一个假设，即过氧化氢酶依赖性代谢的乙醇乙醛在大脑中是一个重要的步骤，在生产乙醇相关的情感特性。首先，我们研究了脑过氧化氢酶在乙醇诱导的条件性位置偏爱（CPP）获得中的作用。其次，用吗啡和可卡因诱导的CPP评价过氧化氢酶抑制剂3-氨基-1，2，4-三唑（AT）的特异性。最后，为了研究过氧化氢酶在由再次暴露于乙醇引起的乙醇寻求复发过程中的作用，在初始条件反射和消退后，用盐水和乙醇或AT和乙醇引发小鼠，并测试CPP的恢复。条件性位置偏爱在AT和乙醇处理的动物中被阻断。吗啡和可卡因CPP不受AT治疗的影响。然而，位置偏爱的恢复并没有被过氧化氢酶抑制所改变。两者合计，本研究的结果表明，大脑过氧化氢酶-H2 O2系统有助于收购乙醇诱导的情感依赖性学习，并支持中央形成的乙醛参与乙醇产生的积极情感记忆的形成。(c)2007年爱思唯尔公司All rights reserved.
It has been suggested that some of the behavioral effects produced by ethanol are mediated by its first metabolite, acetaldehyde. The present research addressed the hypothesis that catalase-dependent metabolism of ethanol to acetaldehyde in the brain is an important step in the production of ethanol-related affective properties. Firstly, we investigated the contribution of brain catalase in the acquisition of ethanol-induced conditioned place preference (CPP). Secondly, the specificity of the catalase inhibitor 3-amino-1,2,4-triazole (AT) was evaluated with morphine- and cocaine-induced CPP. Finally, to investigate the role of catalase in the process of relapse to ethanol seeking caused by re-exposure to ethanol, after an initial conditioning and extinction, mice were primed with saline and ethanol or AT and ethanol and tested for reinstatement of CPP. Conditioned place preference was blocked in animals treated with AT and ethanol. Morphine and cocaine CPP were unaffected by AT treatment. However, the reinstatement of place preference was not modified by catalase inhibition. Taken together, the results of the present study indicate that the brain catalase-H2O2 system contributes to the acquisition of affective-dependent learning induced by ethanol, and support the involvement of centrally-formed acetaldehyde in the formation of positive affective memories produced by ethanol. (c) 2007 Elsevier Inc. All rights reserved.