ASSESSMENT OF PARTICLE RETENTION AND CLEARANCE IN THE INTRAPULMONARY CONDUCTING AIRWAYS OF HAMSTER LUNGS WITH THE FRACTIONATOR
ASSESSMENT OF PARTICLE RETENTION AND CLEARANCE IN THE INTRAPULMONARY CONDUCTING AIRWAYS OF HAMSTER LUNGS WITH THE FRACTIONATOR
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DOI：
10.1111/j.1365-2818.1990.tb03049.x
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发表时间：
1990-10-01
影响因子：
2
通讯作者：
GEHR, P
中科院分区：
文献类型：
--
作者：
GEISER, M;CRUZORIVE, LM;GEHR, P
使用改进的分馏器来估计吸入后两个不同时间点在仓鼠肺的呼吸道中滞留的聚苯乙烯微球的总数。采用系统的三阶段二次抽样程序，抽样比例已知。首先，每个肺被切成切片，从这些切片中，以已知的采样比例系统地对主要病变进行采样。从每个初级样本中，对较小的子样本进行二次抽样，并通过点计数来估计相应的抽样比例。最后，在子偏转器中的微观水平上对几个粒子进行计数，并且总粒子数量的最终估计(无论任何组织变形都是无偏的)很容易被计算为所计数的数量乘以连续采样分数的倒数的乘积。使用新的估计器从数据中评估每个估计值的误差方差。吸入后不久，平均有6%的沉积颗粒滞留在肺内传导通道的上皮表面，其中至少三分之一已被巨噬细胞吞噬。24小时后，平均87%的颗粒物在吸入后不久被清除。在被研究的四只动物中，有三只被巨噬细胞摄取的颗粒比例至少增加到87%。
A modified version of the fractionator was used to estimate the total number of polystyrene microspheres retained in the airways of hamster lungs at two different time points after inhalation. A systematic three-stage subsampling procedure with known sampling fractions was adopted. First, each lung was cut into slices, from which primary disectors were sampled systematically with a known sampling fraction. From each primary disector, smaller sub-disectors were subsampled, and the corresponding sampling fraction was estimated by point counting. Finally, a few particles were counted at the microscopic level in the sub-disectors, and the final estimate of total particle number (which is unbiased irrespective of any tissue deformations) was easily computed as a product of the counted number times the reciprocal of the successive sampling fractions. The error variance of each estimate was assessed from the data using a new estimator. An average of 6% of the deposited particles were retained on the epithelial surface of the intrapulmonary conducting airways shortly after the inhalation, from which at least one-third was already phagocytosed by macrophages. After 24 h, an average of 87% of the particles retained shortly after the inhalation had been cleared. The proportion of particles ingested by macrophages had increased to at least 87% in three out of four animals studied.