Dynamic Imaging of Glucose and Lactate Metabolism by 13C-MRS without Hyperpolarization
Dynamic Imaging of Glucose and Lactate Metabolism by 13C-MRS without Hyperpolarization
复制标题
DOI：
10.1038/s41598-019-38981-1
复制
发表时间：
2019-03-04
影响因子：
4.6
通讯作者：
Krishna, Murali C.
中科院分区：
文献类型：
--
作者：
Brender, Jeffrey R.;Kishimoto, Shun;Krishna, Murali C.
代谢重编程是癌症的定义特征之一，代谢异常与许多其他病理有关。能够检测这种变化的分子成像技术已经成为癌症诊断、治疗计划和监测的必要条件。特别是，F-18-FDG（氟脱氧葡萄糖）PET已成为一种重要的癌症成像方式，因为它具有通过测量葡萄糖摄取来检测干扰分子途径的独特能力。然而，FDG-PET有局限性，限制了它在某些情况下的有用性，并且所获得的信息仅限于葡萄糖摄取。C-13磁共振波谱在理论上比FDG-PET具有一定的优势，但其固有的低灵敏度限制了其主要用于单体素测量，除非使用溶解动态核极化（dDNP）来增加信号，这给临床使用带来了额外的并发症。我们在这里展示了一种通过MRI化学位移成像（CSI）实验来成像体内葡萄糖代谢的新方法，该方法依赖于一种简单，但鲁棒且高效的后处理程序，即通过高维类比的奇异值分解，张量分解。使用此程序，我们在不牺牲精度的情况下，在dDNP和非超极化非定域实验中实现了一个数量级的信噪比增加。在CSI实验中，观察到大约30倍的增加，足以表明葡萄糖到乳酸的转化可以在没有超极化的情况下成像，时间分辨率为12s，总体空间分辨率优于F-18-FDG PET。
Metabolic reprogramming is one of the defining features of cancer and abnormal metabolism is associated with many other pathologies. Molecular imaging techniques capable of detecting such changes have become essential for cancer diagnosis, treatment planning, and surveillance. In particular, F-18-FDG (fluorodeoxyglucose) PET has emerged as an essential imaging modality for cancer because of its unique ability to detect a disturbed molecular pathway through measurements of glucose uptake. However, FDG-PET has limitations that restrict its usefulness in certain situations and the information gained is limited to glucose uptake only. C-13 magnetic resonance spectroscopy theoretically has certain advantages over FDG-PET, but its inherent low sensitivity has restricted its use mostly to single voxel measurements unless dissolution dynamic nuclear polarization (dDNP) is used to increase the signal, which brings additional complications for clinical use. We show here a new method of imaging glucose metabolism in vivo by MRI chemical shift imaging (CSI) experiments that relies on a simple, but robust and efficient, post-processing procedure by the higher dimensional analog of singular value decomposition, tensor decomposition. Using this procedure, we achieve an order of magnitude increase in signal to noise in both dDNP and non-hyperpolarized non-localized experiments without sacrificing accuracy. In CSI experiments an approximately 30-fold increase was observed, enough that the glucose to lactate conversion indicative of the Warburg effect can be imaged without hyper-polarization with a time resolution of 12s and an overall spatial resolution that compares favorably to F-18-FDG PET.