Cytological Analysis of Arabidopsis thaliana Meiotic Chromosomes
Cytological Analysis of Arabidopsis thaliana Meiotic Chromosomes
复制标题
DOI：
10.1007/978-1-60761-103-5_9
复制
发表时间：
2009-01-01
期刊：
影响因子：
--
通讯作者：
Franklin, F. Chris H.
中科院分区：
文献类型：
--
作者：
Armstrong, Susan J.;Sanchez-Moran, Eugenio;Franklin, F. Chris H.
拟南芥分子生物学和遗传学的进步使该生物体成为分析植物减数分裂的重要模型。细胞遗传学研究对于减数分裂研究至关重要，拟南芥细胞学的许多技术改进为研究减数分裂期间的染色体行为提供了一系列工具。本章包括基础细胞学、FISH 分析、免疫细胞学、减数分裂时间过程和电子显微镜的操作方案。
Advances in molecular biology and in the genetics of Arabidopsis thaliana have led to this organism becoming an important model for the analysis of meiosis in plants. Cytogenetic investigations are pivotal to meiotic studies and a number of technological improvements for Arabidopsis cytology have provided a range of tools to investigate chromosome behaviour during meiosis. This chapter includes protocols on basic cytology, FISH analysis, immunocytology, a procedure for a meiotic time course and electron microscopy.