Glass formation and dormancy in bacterial spores
Glass formation and dormancy in bacterial spores
复制标题
DOI：
10.1046/j.1365-2621.1999.00240.x
复制
发表时间：
1999-02-01
影响因子：
3.3
通讯作者：
Martin, DR
中科院分区：
文献类型：
--
作者：
Ablett, S;Darke, AH;Martin, DR
差示扫描量热法（DSC）和核磁共振（NMR）技术被用来获得的证据是否存在于完全水合休眠枯草芽孢杆菌孢子的玻璃态。水合休眠孢子的DSC热谱图包含两个主要转变;一个在类似于60 ° C下被指定为与萌发的初始“活化”阶段相关，另一个在-120 ° C下被指定为与“失活”过程（即杀死孢子）相关。在类似于60 ° C的峰具有全部与处于玻璃态的完全水合休眠孢子的区室化结构的至少一些区域一致的特征。固体（CPMAS）和液体状态（SPMAS）的流动性解决的C-13 NMR光谱研究的钙吡啶二羧酸（CaDPA）的共振，这是只存在于中央原生质体的细菌孢子，表明它是存在于一个无定形的固体样的环境。DSC和NMR的结果都被认为是一致的假设，细菌孢子的休眠是由于至少中央原生质体区域被维持在低水分含量的玻璃态。
Differential scanning calorimetry (DSC) and nuclear magnetic resonance (NMR) techniques were used to obtain evidence whether a glassy state exists within fully hydrated dormant Bacillus subtilis spores. DSC thermograms of hydrated dormant spores contained two major transitions; one at similar to 60 degrees C assigned to be associated with the initial 'activation' stage for germination, and the other at -120 degrees C assigned to be associated with the 'inactivation' process (i.e. killing of the spores). The peak at similar to 60 degrees C had characteristics which were all consistent with at least some region of the compartmentalised structure of fully hydrated dormant spores being in a glassy state. Solid (CPMAS) and liquid state (SPMAS) mobility resolved C-13 NMR spectroscopy studies on the calcium dipicolinic acid (CaDPA) resonances, which are only present in the central protoplast of bacterial spores, showed it to be present in an amorphous solid-like environment. The DSC and NMR results were both found to be consistent with the hypothesis that the dormancy of bacterial spores is due to at least the central protoplast region being maintained in a low moisture content glassy state.