New tools for studying vesicular-mediated protein trafficking: Synthesis and evaluation of ilimaquinone analogs in a non-radioisotope-based antisecretory assay
New tools for studying vesicular-mediated protein trafficking: Synthesis and evaluation of ilimaquinone analogs in a non-radioisotope-based antisecretory assay
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DOI：
10.1021/jo962292l
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发表时间：
1997-05-02
影响因子：
3.6
通讯作者：
Snapper, ML
中科院分区：
文献类型：
--
作者：
Radeke, HS;Digits, CA;Snapper, ML
已经制备了具有可比生物活性的海绵代谢物伊利马醌的结构变体。这些类似物，以及相关的天然产物，通过一种新的，非放射性同位素为基础的分泌测定，筛选其对高尔基体的影响。该测定法已经鉴定了包含多功能接头基团但保留天然产物的细胞活性的伊利马醌的变体。这种功能性的伊利马醌类似物将是研究细胞内蛋白质运输的有价值的工具。
Structural variants of the marine sponge metabolite ilimaquinone, with comparable biological activity, have been prepared. These analogs, as well as related natural products, were screened for their effects on the Golgi apparatus through a novel, non-radioisotope-based secretion assay. The assay has identified a variant of ilimaquinone that contains a versatile linker group yet retains the natural product's cellular activity. This functional ilimaquinone analog will be a valuable tool for studying intracellular protein trafficking.