Imbalanced presence of Borrelia burgdorferi s.l. multilocus sequence types in clinical manifestations of Lyme borreliosis
Imbalanced presence of Borrelia burgdorferi s.l. multilocus sequence types in clinical manifestations of Lyme borreliosis
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DOI：
10.1016/j.meegid.2016.04.019
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发表时间：
2016-08-01
影响因子：
3.2
通讯作者：
Sprong, Hein
中科院分区：
文献类型：
--
作者：
Coipan, E. Claudia;Jahfari, Setareh;Sprong, Hein
本研究采用基于8个多位点序列分型方案的管家基因（MLST）和5S-23 S rDNA基因间间隔区（IGS）的分型方法，探讨莱姆病（LB）患者的伯氏疏螺旋体（Borrelia burgdorferi sensu lato）分离株的群体结构，并检验B与LB的相关性。布格多费里公司序列类型（ST）及其在人类中引起的临床表现。B的分离株。本研究仅分析了来自欧洲183例具有不同临床表现的LB病例的Burgdorferi，和来自荷兰的257份篦麻硬蜱裂解物。为了完整起见，我们在分析中还纳入了335个欧洲B。布格多费里公司从文献中检索MLST曲线。Borrelia afzelii和Borrelia bavariensis与人类LB病例相关，而Borrelia garinii，Borrelia lusitaniae和Borrelia valaisiana与questing I相关。蓖麻蜱。B。Afzelii与慢性萎缩性肢端皮炎相关，而B. garinii和B.与神经疏螺旋体病有关。我们研究中的样本属于251种不同的ST，其中94种是新描述的，增加了疏螺旋体基因种的遗传多样性的总体情况。从人类样本中分离的ST的分数对于已知由哺乳动物维持在地方流行周期中的基因种而言显著更高（B. Afzelii，B. bavariensis和Borrelia spielmanii）的基因种比由鸟类维持的基因种（B. garinii和B. valaisiana）或蜥蜴（B. lusitaniae）。我们发现6个多位点序列类型与人类临床表现显著相关，5个IGS单倍型与人类LB病例相关。而IGS在预测B的感染性方面可以与MLST方案中的管家基因一样好地执行。burgdorferi s.l.，MLST的优点是它还可以捕获各种ST的不同侵袭性。(C)2016年6月，作者。由Elsevier B出版。诉
In this study we used typing based on the eight multilocus sequence typing scheme housekeeping genes (MLST) and 5S-23S rDNA intergenic spacer (IGS) to explore the population structure of Borrelia burgdorferi sensu lato isolates frompatients with Lyme borreliosis (LB) and to test the association between the B. burgdorferi s.l. sequence types (ST) and the clinical manifestations they cause in humans. Isolates of B. burgdorferi from 183 LB cases across Europe, with distinct clinical manifestations, and 257 Ixodes ricinus lysates from The Netherlands, were analyzed for this study alone. For completeness, we incorporated in our analysis also 335 European B. burgdorferi s.l. MLST profiles retrieved from literature. Borrelia afzelii and Borrelia bavariensis were associated with human cases of LB while Borrelia garinii, Borrelia lusitaniae and Borrelia valaisiana were associated with questing I. ricinus ticks. B. afzelii was associated with acrodermatitis chronica atrophicans, while B. garinii and B. bavariensis were associated with neuroborreliosis. The samples in our study belonged to 251 different STs, of which 94 are newly described, adding to the overall picture of the genetic diversity of Borrelia genospecies. The fraction of STs that were isolated from human samples was significantly higher for the genospecies that are known to be maintained in enzootic cycles by mammals (B. afzelii, B. bavariensis, and Borrelia spielmanii) than for genospecies that are maintained by birds (B. garinii and B. valaisiana) or lizards (B. lusitaniae). We found six multilocus sequence types that were significantly associated to clinical manifestations in humans and five IGS haplotypes that were associated with the human LB cases. While IGS could perform just as well as the housekeeping genes in the MLST scheme for predicting the infectivity of B. burgdorferi s.l., the advantage of MLST is that it can also capture the differential invasiveness of the various STs. (C) 2016 The Authors. Published by Elsevier B. V.