Study of vitreous liquifaction by NMR spectroscopy and imaging.
Study of vitreous liquifaction by NMR spectroscopy and imaging.
复制标题
通过核磁共振波谱和成像研究玻璃体液化。
DOI：
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发表时间：
1985
影响因子：
4.4
通讯作者：
T. Brady
中科院分区：
文献类型：
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作者：
J. Aguayo;B. Glaser;A. Mildvan;H. Cheng;R. G. González;T. Brady
玻璃体凝胶主要由胶原蛋白、透明质酸和水 (98-99%) 组成，可以分解成不含胶原蛋白的液态。随着年龄的增长和某些其他疾病状态发生的玻璃体凝胶液化被认为在视网膜撕裂和脱离的发病机制中很重要。作者报告了水质子弛豫时间和水质子核磁共振（NMR）成像的测量结果，以研究提取的玻璃体和完整牛眼中的玻璃体液化过程。对 NMR 波谱仪获得的宏观粘度、纵向 (T1) 和横向 (T2) 弛豫时间以及质子 NMR 图像进行比较。用胶原酶处理玻璃体并用透明质酸酶处理玻璃体后，导致玻璃体液化（通过宏观粘度的降低来测量）。松弛时间的缩短伴随着粘度的下降。仅在注射胶原酶后，通过 NMR 成像才能看到与液化焦点相对应的亮度或质子信号强度增加的区域。作者相信核磁共振成像是一种有用的新诊断方式，可以在完整的眼睛中研究玻璃体液化。玻璃体为研究生物系统中蛋白质溶液的质子弛豫时间的变化提供了一种新模型。
The vitreous gel is primarily composed of collagen, hyaluronic acid, and water (98-99%) and can break down into a liquid state devoid of collagen. The liquifaction of vitreous gel that occurs with age and in certain other disease states is believed to be important in the pathogenesis of retinal tears and detachments. The authors report measurements of water proton relaxation times and water proton nuclear magnetic resonance (NMR) imaging to study the process of vitreous liquifaction in extracted vitreous and in the intact bovine eye. A comparison is made between macroscopic viscosity, longitudinal (T1) and transverse (T2) relaxation times obtained on an NMR spectrometer and proton NMR images. Vitreous liquifaction as measured by a decrease in macroscopic viscosity resulted after treatment of vitreous with collagenase and to lesser degree with hyaluronidase. A shortening of relaxation times accompanied the drop in viscosity. An area of brightness or increased proton signal intensity corresponding to a focus of liquifaction was seen by NMR imaging only after injection with collagenase. The authors believe NMR imaging to be a useful new diagnostic modality by which vitreous liquifaction can be studied in the intact eye. The vitreous provides a new model for studying changes in proton relaxation times of protein solutions in biologic systems.