Early and transient ontogenetic expression of the cocaine- and amphetamine-regulated transcript peptide in the rat mesencephalon: Correlation with tyrosine hydroxylase expression
Early and transient ontogenetic expression of the cocaine- and amphetamine-regulated transcript peptide in the rat mesencephalon: Correlation with tyrosine hydroxylase expression
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DOI：
10.1002/neu.10077
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发表时间：
2002-09-05
期刊：
影响因子：
--
通讯作者：
Risold, PY
中科院分区：
文献类型：
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作者：
Brischoux, F;Griffond, B;Risold, PY
用免疫组织化学方法分析了可卡因和苯丙胺调节转录物(CART)在大鼠中枢神经系统中的表达，并与酪氨酸羟化酶(TH-)的表达模式进行了比较。在胚胎第11天(E11)，在腹侧中脑囊泡中首次检测到产生CART的神经元。这些神经元是最早分化的外套层细胞之一。从E13开始，观察到一种互补的分布模式，将地幔分为外部TH带和内部CART带。发现许多TH阳性神经元从神经上皮细胞通过含有CART免疫反应神经元的区域迁移到更外侧的安置区。这些TH细胞在CART PERKARYA附近显示明显的前导树突和拖尾树突。在E16，CART神经元的数量似乎减少，它们局限在脑室附近和反屈束周围。在E18和E20，只有Edinger-Westphal核显示出与成人大脑相同的CART染色。因此，在产前个体发育过程中很早就发现了CART，这让我们推测这种多肽可能在大鼠大脑特定区域的发育中发挥作用。特别是，我们的观察表明，表达CART的神经元可能有助于黑质多巴胺能神经元的迁移。(C)2002年威利期刊公司。
The ontogeny of cocaine- and amphetamine-regulated transcript (CART) expression has been analyzed by immunohistochemistry in the mesencephalon of the rat central nervous system, and compared to the pattern of tyrosine hydroxylase- (TH-) expression. CART-producing neurons were first detected on the embryonic day 11 (Ell) in the ventral mesencephalic vesicle. These neurons are among the first cells of the mantle layer to differentiate. From E13, a complementary pattern of distribution was observed, dividing the mantle layer into an external TH zone and an internal CART zone. Many TH-positive neurons were found to migrate from the neuroepithelium through the area containing the CART-immunoreactive neurons to settle more laterally. These TH cells exhibited prominent leading and trailing dendrites in the immediate vicinity of CART perikarya. On E16, the number of CART neurons appeared to diminish, and they were confined near the ventricle and around the fasciculus retroflexus. On E18 and E20, only the Edinger-Westphal nucleus exhibited a strong CART staining as described in the adult brain. Thus, the very early detection of CART during prenatal ontogeny led us to speculate that this peptide might have a role in the development of specific regions of the rat brain. In particular, our observations suggest that CART-expressing neurons might help the migration of the dopaminergic neurons of the substantia nigra. (C) 2002 Wiley Periodicals, Inc.