The role of phytosterols in plant adaptation to temperature
The role of phytosterols in plant adaptation to temperature
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DOI：
10.4161/psb.3.2.5051
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发表时间：
2008-01-01
影响因子：
2.9
通讯作者：
Dufourc, Erick J.
中科院分区：
文献类型：
--
作者：
Dufourc, Erick J.
用固体~ 2 H-NMR研究了组成接近植物、真菌和哺乳动物“筏”的膜，用氘代二棕榈酰磷脂酰胆碱（~ 2 H-DPPC）作报告分子。这种膜的动力学通过测量膜的有序或无序性质来确定。在所有情况下，检测到的液体有序，LO相，作为一个指标的刚性甾醇鞘脂结构域的存在。非常感兴趣的是，在植物中模仿筏的混合物的动力学表现出较低的温度敏感性热冲击。在主要植物甾醇（谷甾醇和豆甾醇）的烷基链上支链的额外乙基的存在被提议为增强膜内聚力。因此，甾醇结构的微调似乎是植物适应大的温度变化的进化反应。
Membranes of composition approaching those found in "rafts" of plants, fungi and mammals were investigated by means of solidstate 2H-NMR, using deuterated dipalmitoyl-phosphatidylcholine (2H-DPPC) as a reporter. The dynamics of such membranes was determined through measuring of membrane ordering or disordering properties. The presence of the liquid-ordered, lo, phase, as an indicator of rigid sterol-sphingolipid domains, was detected in all cases. Of great interest, the dynamics of mixtures mimicking rafts in plants showed the lesser temperature sensitivity to thermal shocks. The presence of an additional ethyl group branched on the alkyl chain of major plant sterols (sitosterol and stigmasterol) is proposed as reinforcing the membrane cohesion. The fine tuning of the sterol structure thus appears to be the evolution response for plant adaptation to large temperature variations.