A protein import receptor in pea chloroplasts, Toc86, is only a proteolytic fragment of a larger polypeptide
A protein import receptor in pea chloroplasts, Toc86, is only a proteolytic fragment of a larger polypeptide
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DOI：
10.1016/s0014-5793(98)01525-7
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发表时间：
1998-12-11
期刊：
影响因子：
3.5
通讯作者：
Soll, J
中科院分区：
文献类型：
--
作者：
Bölter, B;May, T;Soll, J
叶绿体外被膜的蛋白质输入复合物（Toc-复合物）含有一个显著的86 kDa分子量的亚基（Toc 86）。Toc 86被鉴定为推定的前体受体。拟南芥基因组测序计划表明，Toc 86只代表一个较大的多肽160 kDa的蛋白水解片段。160-kDa的蛋白质，我们命名为Toc 160，只存在于严格条件下分离的豌豆叶绿体中的显着量。Toc 160的完整性与叶绿体转运前体蛋白的能力密切相关。我们认为Toc 160仍然是叶绿体转运前体蛋白的一个真正的亚基。(C)1998年欧洲生物化学学会联合会。
The protein import complex of the chloroplastic outer envelope (Toc-complex) contains a prominent subunit of 86 kDa molecular weight (Toc86). Toc86 was identified as a putative precursor receptor. The Arabidopsis genome sequencing project indicates that Toc86 represents only a proteolytic fragment of a larger polypeptide of 160 kDa. The 160-kDa protein, which we name Toc160, is only present in significant amounts in pea chloroplasts isolated under stringent conditions. The capacity of chloroplasts to import an in vitro translated precursor protein correlates well with the integrity of Toc160, We conclude that Toc160 is still a bonafide subunit of the protein import machinery of chloroplasts. (C) 1998 Federation of European Biochemical Societies.