Efficient protoplast regeneration for some homofermentative lactobacilli and pediococci
Efficient protoplast regeneration for some homofermentative lactobacilli and pediococci
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DOI：
10.1007/s00203-001-0357-3
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发表时间：
2001-10
影响因子：
2.8
通讯作者：
O. Tanaka;S. Ohmomo
中科院分区：
文献类型：
--
作者：
O. Tanaka;S. Ohmomo
Conditions for protoplast regeneration were examined for several strains of homofermentative lactobacilli and pediococci isolated from silage. Attempts to regenerate protoplasts using previously published agar regeneration media for lactobacilli were unsuccessful for most of the strains. Replacing or increasing colloidal substances in a medium containing raffinose and MgCl2as osmotic stabilizers enabled efficient regeneration of the protoplasts at a frequency of 10–99%. A medium containing gelatin, polyvinylpyrrolidone (PVP) and no agar was effective forLactobacillus plantarum,Lactobacillus pentosusandLactobacillus rhamnosusprotoplasts. An agar medium containing PVP (PVP medium) was effective forPediococcussp. protoplasts, and addition of agarose to the PVP medium enabled regeneration ofLactobacillus caseiprotoplasts. A medium containing calcium alginate gel and no agar was effective forLactobacillus curvatusprotoplasts. The type of colloidal substance required for protoplast regeneration varied from species to species. This result suggested that several kinds of media may be necessary to regenerate protoplasts for all the genera of lactobacilli and pediococci.
